Phenotype and function of hematopoietic stem cells

Saul J. Sharkis; Sara Neutzel; Michael I. Collector

doi:10.1111/j.1749-6632.2001.tb03589.x

Phenotype and function of hematopoietic stem cells

Saul J. Sharkis, Sara Neutzel, Michael I. Collector

School of Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Using an in vivo selection technique, we can isolate individual cells that can repopulate the hematopoietic system of a lethally irradiated murine recipient. These cells rapidly acquire a CD34 phenotype in the animal. Progenitors in our long-term chimeras are of donor type. We also have evidence that transplantation of limiting numbers (as low as a single cell) that have this long-term repopulating ability (LTRA) can self-renew. This is demonstrated by serial transplantation of marrow from engrafted recipients 11 months post transplant into new hosts for four additional months.

Original language	English (US)
Pages (from-to)	191-195
Number of pages	5
Journal	Annals of the New York Academy of Sciences
Volume	938
DOIs	https://doi.org/10.1111/j.1749-6632.2001.tb03589.x
State	Published - 2001

Keywords

Self-renewal
Single stem cell transplantation
Stem cell homing

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
History and Philosophy of Science

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AU - Sharkis, Saul J.

AU - Neutzel, Sara

AU - Collector, Michael I.

PY - 2001

Y1 - 2001

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AB - Using an in vivo selection technique, we can isolate individual cells that can repopulate the hematopoietic system of a lethally irradiated murine recipient. These cells rapidly acquire a CD34 phenotype in the animal. Progenitors in our long-term chimeras are of donor type. We also have evidence that transplantation of limiting numbers (as low as a single cell) that have this long-term repopulating ability (LTRA) can self-renew. This is demonstrated by serial transplantation of marrow from engrafted recipients 11 months post transplant into new hosts for four additional months.

KW - Self-renewal

KW - Single stem cell transplantation

KW - Stem cell homing

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JO - Annals of the New York Academy of Sciences

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Phenotype and function of hematopoietic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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