Pharmacokinetics and pharmacodynamics of five distinct commercially available hemp-derived topical cannabidiol products

C. Austin Zamarripa, Hayleigh E. Tilton, Spencer Lin, Edward Jackson Cone, Ruth E. Winecker, Ronald R. Flegel, David Kuntz, Melissa Beals, Martin Jacques, Michael Clark, Eric R. Welsh, Lynn Wagner, Marcel O. Bonn-Miller, Ryan Vandrey, Tory R. Spindle

Research output: Contribution to journalArticlepeer-review

Abstract

Products containing cannabidiol (CBD) have proliferated after the 2018 Farm Bill legalized hemp (cannabis with ≤0.3% delta-9tetrahydrocannabinol (Δ9-THC)). CBD-containing topical products have surged in popularity, but controlled clinical studies on them are limited. This study characterized the effects of five commercially available hemp-derived high CBD/low Δ9-THC topical products. Healthy adults (N= 46) received one of six study drugs: a CBD-containing cream (N = 8), lotion (N = 8), patch (N = 7), balm (N= 8), gel (N = 6) or placebo (N= 9; matched to an active formulation).The protocol included three phases conducted over 17 days: (i) an acute drug application laboratory session, (ii) a 9-day outpatient phase with twice daily product application (visits occurred on Days 2, 3, 7 and 10) (iii) a 1-week washout phase. In each phase, whole blood, oral fluid and urine specimens were collected and analyzed via liquid chromatography with tandem mass spectrometry (LC–MS-MS) for CBD, Δ9-THC and primary metabolites of each and pharmacodynamic outcomes (subjective, cognitive/psychomotor and physiological effects) were assessed.Transdermal absorption of CBD was observed for three active products. On average, CBD/metabolite concentrations peaked after 7–10 days of product use and were highest for the lotion, which contained the most CBD and a permeation enhancer (vitamin E). Δ9-THC/metabolites were below the limit of detection in blood for all products, and no urine samples tested “positive” for cannabis using current US federal workplace drug testing criteria (immunoassay cut-off of 50 ng/mL and confirmatory LC–MS-MS cut-off of 15 ng/mL). Unexpectedly, nine participants (seven lotions, one patch and one gel) exhibited Δ9-THC oral fluid concentrations ≥2 ng/mL (current US federal workplace threshold for a “positive” test). Products did not produce discernable pharmacodynamic effects and were well-tolerated. This study provides important initial data on the acute/chronic effects of hemp-derived topical CBD products, but more research is needed given the diversity of products in this market.

Original languageEnglish (US)
Pages (from-to)81-98
Number of pages18
JournalJournal of analytical toxicology
Volume48
Issue number2
DOIs
StatePublished - Mar 1 2024

ASJC Scopus subject areas

  • General Medicine

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