TY - JOUR
T1 - Percentage binding of testosterone, androstenedione and dehydroisoandrosterone in human plasma
AU - Forest, Maguelone G.
AU - Rivarola, Marco A.
AU - Migeon, Claude J.
N1 - Funding Information:
Supported by USPHS Research Grant AM-00180 and Research Career award K6-AM-21, 855 (CJM), by The John Simon G~g~eim Memori~ Foundation and Consejo National de Investigaoiones Cientificas y Tecnicas of Argentina (MAR), and by Institut National de la Santk et de la Recherche Medicale and Direction G&&ale des Affaires Culturelles et Techniques of France (MGF). Grateful appreciation is also due to Miss Kathleen R. Scheller for her secretarial assistance.
PY - 1968/9
Y1 - 1968/9
N2 - Equilibrium dialysis, ultrafiltration by centrifugation and by vacuum gave similar results for the percentage binding of testosterone (T), androstenedione (Δ) and dehydroisoandrosterone (D) when using plasma samples diluted 1:5 at 37°C. The percentage binding of T is significantly lower in male plasma than in female plasma. Pregnancy and estrogen treatment in males increase T binding, whereas ProveraRtreatment in males decreases it. In addition to T itself, 5α-androstan-3α, 17β-diol, estradiol-17β and to a smaller extent estrone can compete for T binding. The percentage binding of Δ was similar in male and female plasma. Pregnancy and estrogen or ProveraR treatment in males had no effect. In range of concentration used, there was no binding competition by the steroid tested. Although the percentage binding of D was not influenced by sex, it was increased in pregnant women and estrogen-treated men. There was also a slight competition by T.
AB - Equilibrium dialysis, ultrafiltration by centrifugation and by vacuum gave similar results for the percentage binding of testosterone (T), androstenedione (Δ) and dehydroisoandrosterone (D) when using plasma samples diluted 1:5 at 37°C. The percentage binding of T is significantly lower in male plasma than in female plasma. Pregnancy and estrogen treatment in males increase T binding, whereas ProveraRtreatment in males decreases it. In addition to T itself, 5α-androstan-3α, 17β-diol, estradiol-17β and to a smaller extent estrone can compete for T binding. The percentage binding of Δ was similar in male and female plasma. Pregnancy and estrogen or ProveraR treatment in males had no effect. In range of concentration used, there was no binding competition by the steroid tested. Although the percentage binding of D was not influenced by sex, it was increased in pregnant women and estrogen-treated men. There was also a slight competition by T.
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U2 - 10.1016/0039-128X(68)90025-1
DO - 10.1016/0039-128X(68)90025-1
M3 - Article
C2 - 4233917
AN - SCOPUS:0014333227
SN - 0039-128X
VL - 12
SP - 323
EP - 343
JO - Steroids
JF - Steroids
IS - 3
ER -