TY - JOUR
T1 - Peptidomic Analysis of Maternal Serum to Identify Biomarker Candidates for Prenatal Diagnosis of Tetralogy of Fallot
AU - Zhuang, Bin
AU - Hu, Yin
AU - Fan, Xuemei
AU - Li, Mengmeng
AU - Zhu, Jingai
AU - Liu, Heng
AU - Cao, Li
AU - Liang, Dong
AU - Zhang, Jingjing
AU - Yu, Zhangbin
AU - Han, Shuping
N1 - Funding Information:
Abbreviations used: ACN, acetonitrile; CHD, congenital heart disease; IPA, ingenuity pathway analysis; kDa, kilo Dalton; LC/MS, liquid chromatography/mass spectrometry; LMW, low molecular weight; RV, (right ventricular) outflow tract obstruction; RVH, right ventricular hypertrophy; TOF, Tetralogy of Fallot; VSD, ventricular septal defect. Bin Zhuang and Yin Hu contributed equally to this work. Disclosure statement: The authors have declared no conflict of interest. Grant sponsor: Nanjing Medical Science and Technique Development Foundation; Grant number: 201605052; Grant sponsor: National Natural Science Foundation of Jiangsu Province of China; Grant number: BK20141077; Grant sponsor: National Natural Science Foundation of China; Grant number: 81370200. *Correspondence to: Prof. Shuping Han and Prof. Zhangbin Yu, Department of Pediatrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, 123rd Tian Fei Xiang, Mo Chou Road, Nanjing 210004, Jiangsu Province, China. E-mails: [email protected] (S.H.) and [email protected] (Z.Y.) Manuscript Received: 20 April 2017; Manuscript Accepted: 8 June 2017 Accepted manuscript online in Wiley Online Library (wileyonlinelibrary.com): 9 June 2017 DOI 10.1002/jcb.26204 • © 2017 Wiley Periodicals, Inc.
Funding Information:
This study was supported by grants from the National Natural Science Foundation of China (grant no. 81370200), the National Natural Science Foundation of Jiangsu Province of China (No. BK20141077), and the Nanjing Medical Science and Technique Development Foundation (No. 201605052).
Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
PY - 2018/1
Y1 - 2018/1
N2 - Tetralogy of Fallot (TOF) is the most common form of cyanotic congenital heart disease. To identify endogenous peptides possibly involved in the progression of TOF, we performed comparative peptidomic profiling of maternal serum between normal fetuses and fetuses suffering from TOF. A total of 278 differentially expressed peptides, including 94 over-expressed peptides and 184 under-expressed peptides, originating from 227 protein precursors were identified by liquid chromatography/mass spectrometry (LC/MS) in maternal serum of fetuses with TOF compared to normal controls. Further, ingenuity pathway analysis (IPA) was used to identify putative roles for these peptides in cardiovascular development. Two peptides were derived from functional domains of proteins involved in heart development and associated with TOF; these may represent candidate bioactive peptides involved in TOF. These peptides may be related to the pathologic changes in the heart associated with TOF, and may be useful as novel biomarkers for prenatal diagnosis of TOF. J. Cell. Biochem. 119: 468–477, 2018.
AB - Tetralogy of Fallot (TOF) is the most common form of cyanotic congenital heart disease. To identify endogenous peptides possibly involved in the progression of TOF, we performed comparative peptidomic profiling of maternal serum between normal fetuses and fetuses suffering from TOF. A total of 278 differentially expressed peptides, including 94 over-expressed peptides and 184 under-expressed peptides, originating from 227 protein precursors were identified by liquid chromatography/mass spectrometry (LC/MS) in maternal serum of fetuses with TOF compared to normal controls. Further, ingenuity pathway analysis (IPA) was used to identify putative roles for these peptides in cardiovascular development. Two peptides were derived from functional domains of proteins involved in heart development and associated with TOF; these may represent candidate bioactive peptides involved in TOF. These peptides may be related to the pathologic changes in the heart associated with TOF, and may be useful as novel biomarkers for prenatal diagnosis of TOF. J. Cell. Biochem. 119: 468–477, 2018.
KW - BIOMARKER
KW - MATERNAL SERUM
KW - PEPTIDOMIC ANALYSIS
KW - TETRALOGY OF FALLOT
UR - https://www.scopus.com/pages/publications/85034781032
UR - https://www.scopus.com/pages/publications/85034781032#tab=citedBy
U2 - 10.1002/jcb.26204
DO - 10.1002/jcb.26204
M3 - Article
C2 - 28598000
AN - SCOPUS:85034781032
SN - 0730-2312
VL - 119
SP - 468
EP - 477
JO - Journal of cellular biochemistry
JF - Journal of cellular biochemistry
IS - 1
ER -