pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland

Partha Seshaiah; Bret Miller; Monn Monn Myat; Deborah J. Andrew

doi:10.1006/dbio.2001.0429

pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland

Partha Seshaiah, Bret Miller, Monn Monn Myat, Deborah J. Andrew

School of Medicine

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.

Original language	English (US)
Pages (from-to)	309-322
Number of pages	14
Journal	Developmental biology
Volume	239
Issue number	2
DOIs	https://doi.org/10.1006/dbio.2001.0429
State	Published - Nov 15 2001

Keywords

Drosophila
KH-domain
Morphogenesis
Nova-1
Nova-2
POMA
RNA-binding protein
Salivary gland
Secretion
Splicing

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1006/dbio.2001.0429

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title = "pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland",

abstract = "From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.",

keywords = "Drosophila, KH-domain, Morphogenesis, Nova-1, Nova-2, POMA, RNA-binding protein, Salivary gland, Secretion, Splicing",

author = "Partha Seshaiah and Bret Miller and Myat, {Monn Monn} and Andrew, {Deborah J.}",

note = "Funding Information: We thank Elliott Abrams, Pamela Bradley, Melissa Vining, and Wendy Yee for their critical comments on the manuscript. We thank Corey Goodman for the ETps (N33) line and the Indiana Drosophila Stock Center for deficiencies in the 85D region. Support for this research was provided by Grant 1RO1DE013899 from the National Institute of Dental and Craniofacial Research (to D.J.A.) and by a Jane Coffin Childs postdoctoral fellowship (to M.M.M.).",

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journal = "Developmental biology",

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AU - Miller, Bret

AU - Myat, Monn Monn

AU - Andrew, Deborah J.

N1 - Funding Information: We thank Elliott Abrams, Pamela Bradley, Melissa Vining, and Wendy Yee for their critical comments on the manuscript. We thank Corey Goodman for the ETps (N33) line and the Indiana Drosophila Stock Center for deficiencies in the 85D region. Support for this research was provided by Grant 1RO1DE013899 from the National Institute of Dental and Craniofacial Research (to D.J.A.) and by a Jane Coffin Childs postdoctoral fellowship (to M.M.M.).

PY - 2001/11/15

Y1 - 2001/11/15

N2 - From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.

AB - From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.

KW - Drosophila

KW - KH-domain

KW - Morphogenesis

KW - Nova-1

KW - Nova-2

KW - POMA

KW - RNA-binding protein

KW - Salivary gland

KW - Secretion

KW - Splicing

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pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland

Abstract

Keywords

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