Partitioning circadian transcription by SIRT6 leads to segregated control of cellular metabolism

Selma Masri, Paul Rigor, Marlene Cervantes, Nicholas Ceglia, Carlos Sebastian, Cuiying Xiao, Manuel Roqueta-Rivera, Chuxia Deng, Timothy F. Osborne, Raul Mostoslavsky, Pierre Baldi, Paolo Sassone-Corsi

Research output: Contribution to journalArticlepeer-review

162 Scopus citations


Circadian rhythms are intimately linked to cellular metabolism. Specifically, the NAD+-dependent deacetylase SIRT1, the founding member of the sirtuin family, contributes to clock function. Whereas SIRT1 exhibits diversity in deacetylation targets and subcellular localization, SIRT6 is the only constitutively chromatin-associated sirtuin and is prominently present at transcriptionally active genomic loci. Comparison of the hepatic circadian transcriptomes reveals that SIRT6 and SIRT1 separately control transcriptional specificity and therefore define distinctly partitioned classes of circadian genes. SIRT6 interacts with CLOCK:BMAL1 and, differently from SIRT1, governs their chromatin recruitment to circadian gene promoters. Moreover, SIRT6 controls circadian chromatin recruitment of SREBP-1, resulting in the cyclic regulation of genes implicated in fatty acid and cholesterol metabolism. This mechanism parallels a phenotypic disruption in fatty acid metabolism in SIRT6 null mice as revealed by circadian metabolome analyses. Thus, genomic partitioning by two independent sirtuins contributes to differential control of circadian metabolism.

Original languageEnglish (US)
Pages (from-to)659-672
Number of pages14
Issue number3
StatePublished - Jul 31 2014
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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