Partial denaturation of Haemophilus influenzae DNA by alkali

Alan D. Cooper; P. C. Huang

doi:10.1016/0005-2787(71)90084-0

Partial denaturation of Haemophilus influenzae DNA by alkali

Alan D. Cooper, P. C. Huang

Bloomberg School of Public Health

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Abstract

Suboptimal condition of alkaline denaturation was used to partially denature Haemophilus influenzae DNA and fractions from these DNA molecules possessing various degrees of strandedness were resolved by hydroxyapatite column chromatography. Exonuclease I sensitivity as well as transforming activity were used to elucidate the secondary structure of these fractions. Fractions supposedly corresponding to the single- and double-stranded DNA were shown to be different from the completely denatured and native DNA, respectively.

Original language	English (US)
Pages (from-to)	180-185
Number of pages	6
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	238
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(71)90084-0
State	Published - May 13 1971

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Medicine(all)

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abstract = "Suboptimal condition of alkaline denaturation was used to partially denature Haemophilus influenzae DNA and fractions from these DNA molecules possessing various degrees of strandedness were resolved by hydroxyapatite column chromatography. Exonuclease I sensitivity as well as transforming activity were used to elucidate the secondary structure of these fractions. Fractions supposedly corresponding to the single- and double-stranded DNA were shown to be different from the completely denatured and native DNA, respectively.",

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AB - Suboptimal condition of alkaline denaturation was used to partially denature Haemophilus influenzae DNA and fractions from these DNA molecules possessing various degrees of strandedness were resolved by hydroxyapatite column chromatography. Exonuclease I sensitivity as well as transforming activity were used to elucidate the secondary structure of these fractions. Fractions supposedly corresponding to the single- and double-stranded DNA were shown to be different from the completely denatured and native DNA, respectively.

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Partial denaturation of Haemophilus influenzae DNA by alkali

Abstract

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