TY - JOUR
T1 - Partial Allelotype of Carcinoma in Situ of the Human Bladder
AU - Rosin, Miriam P.
AU - Cairns, Paul
AU - Sidransky, David
AU - Epstein, J. I.
AU - Schoenberg, Mark P.
PY - 1995/11/15
Y1 - 1995/11/15
N2 - Carcinoma in situ (CIS) of the urinary bladder is an aggressive lesion that frequently progresses to an invasive tumor, yet the underlying molecular changes in this lesion are largely unknown. In this study, we microdissected 31 cases of CIS and examined them for loss of heterozygosity (LOH) on 13 chromosomal arms. Twenty-nine microsatellite markers were chosen for this analysis based on their location in regions previously shown to be frequently lost in primary transitional cell carcinoma of the bladder. LOH of chromosome 9 was a frequent event in these samples, occurring in 77% of these lesions, with 19 of 31 cases showing deletion on the 9p arm (61%) and 17 of 28 cases displaying LOH on 9q (61 %). Fine mapping at 9p21 demonstrated that CIS also displayed a high frequency of homozygous deletion surrounding the pl6,NK4A locus, like superficial papillary tumors, the other form of noninvasive lesion found in the bladder. However, loss of 14q (70%) was frequent in CIS yet extremely rare in papillary lesions (9%). Other chromosomal arms showing frequent LOH included 8p (65%), 17p (60%), 13q (56%), lip (54%), and 4q (52%), whereas slightly lower frequencies of loss were observed for llq (36%), 4p (32%), 3p (31%), 18q (29%), and 5q (20%). CIS lesions already possess many of the genetic alterations displayed by invasive transitional cell carcinomas, potentially accounting for the aggressive nature of these lesions.
AB - Carcinoma in situ (CIS) of the urinary bladder is an aggressive lesion that frequently progresses to an invasive tumor, yet the underlying molecular changes in this lesion are largely unknown. In this study, we microdissected 31 cases of CIS and examined them for loss of heterozygosity (LOH) on 13 chromosomal arms. Twenty-nine microsatellite markers were chosen for this analysis based on their location in regions previously shown to be frequently lost in primary transitional cell carcinoma of the bladder. LOH of chromosome 9 was a frequent event in these samples, occurring in 77% of these lesions, with 19 of 31 cases showing deletion on the 9p arm (61%) and 17 of 28 cases displaying LOH on 9q (61 %). Fine mapping at 9p21 demonstrated that CIS also displayed a high frequency of homozygous deletion surrounding the pl6,NK4A locus, like superficial papillary tumors, the other form of noninvasive lesion found in the bladder. However, loss of 14q (70%) was frequent in CIS yet extremely rare in papillary lesions (9%). Other chromosomal arms showing frequent LOH included 8p (65%), 17p (60%), 13q (56%), lip (54%), and 4q (52%), whereas slightly lower frequencies of loss were observed for llq (36%), 4p (32%), 3p (31%), 18q (29%), and 5q (20%). CIS lesions already possess many of the genetic alterations displayed by invasive transitional cell carcinomas, potentially accounting for the aggressive nature of these lesions.
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M3 - Article
C2 - 7585577
AN - SCOPUS:0028849278
SN - 0008-5472
VL - 55
SP - 5213
EP - 5216
JO - Cancer Research
JF - Cancer Research
IS - 22
ER -