Abstract
Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data. This method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.
Original language | English (US) |
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Pages (from-to) | 1451-1456 |
Number of pages | 6 |
Journal | Journal of proteome research |
Volume | 4 |
Issue number | 4 |
DOIs | |
State | Published - Jul 2005 |
Externally published | Yes |
Keywords
- DNA-binding activity
- EMSA
- Oligonucleotide array
- Oligonucleotide array-based transcription factor assay (OATFA)
- Transcription factor
ASJC Scopus subject areas
- General Chemistry
- Biochemistry