Oxygen consumption of human blood platelets. I. Effect of thrombin

Joseph Muenzer; Eugene C. Weinbach; Sidney M. Wolfe

doi:10.1016/0005-2728(75)90015-8

Oxygen consumption of human blood platelets. I. Effect of thrombin

Joseph Muenzer, Eugene C. Weinbach, Sidney M. Wolfe

Bloomberg School of Public Health

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18 Scopus citations

Abstract

The effect of thrombin on the oxygen consumption of washed human platelets was measured polarographically with the Clark oxygen electrode. The average basal respiratory rate was 18±1.6 (mean ±S.E.) natoms oxygen per min per 10⁹ platelets. Thrombin (1.9 units/ml) caused a 4-13-fold increase in the rate of oxygen consumption (138±14 (mean ±S.E.) natoms oxygen per min per 10⁹ platelets). The thrombin-stimulated increase of oxygen consumption was transient, lasting from 1 to 1.5 min before returning to the respiratory rate observed before the thrombin addition. Release of platelet constituents appeared to precede the stimulation of oxygen consumption. These results may provide a basis for explaining the discrepancy in the literature concerning the effects of thrombin on platelet respiration.

Original language	English (US)
Pages (from-to)	237-242
Number of pages	6
Journal	BBA - Bioenergetics
Volume	376
Issue number	2
DOIs	https://doi.org/10.1016/0005-2728(75)90015-8
State	Published - Feb 17 1975

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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abstract = "The effect of thrombin on the oxygen consumption of washed human platelets was measured polarographically with the Clark oxygen electrode. The average basal respiratory rate was 18±1.6 (mean ±S.E.) natoms oxygen per min per 109 platelets. Thrombin (1.9 units/ml) caused a 4-13-fold increase in the rate of oxygen consumption (138±14 (mean ±S.E.) natoms oxygen per min per 109 platelets). The thrombin-stimulated increase of oxygen consumption was transient, lasting from 1 to 1.5 min before returning to the respiratory rate observed before the thrombin addition. Release of platelet constituents appeared to precede the stimulation of oxygen consumption. These results may provide a basis for explaining the discrepancy in the literature concerning the effects of thrombin on platelet respiration.",

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N2 - The effect of thrombin on the oxygen consumption of washed human platelets was measured polarographically with the Clark oxygen electrode. The average basal respiratory rate was 18±1.6 (mean ±S.E.) natoms oxygen per min per 109 platelets. Thrombin (1.9 units/ml) caused a 4-13-fold increase in the rate of oxygen consumption (138±14 (mean ±S.E.) natoms oxygen per min per 109 platelets). The thrombin-stimulated increase of oxygen consumption was transient, lasting from 1 to 1.5 min before returning to the respiratory rate observed before the thrombin addition. Release of platelet constituents appeared to precede the stimulation of oxygen consumption. These results may provide a basis for explaining the discrepancy in the literature concerning the effects of thrombin on platelet respiration.

AB - The effect of thrombin on the oxygen consumption of washed human platelets was measured polarographically with the Clark oxygen electrode. The average basal respiratory rate was 18±1.6 (mean ±S.E.) natoms oxygen per min per 109 platelets. Thrombin (1.9 units/ml) caused a 4-13-fold increase in the rate of oxygen consumption (138±14 (mean ±S.E.) natoms oxygen per min per 109 platelets). The thrombin-stimulated increase of oxygen consumption was transient, lasting from 1 to 1.5 min before returning to the respiratory rate observed before the thrombin addition. Release of platelet constituents appeared to precede the stimulation of oxygen consumption. These results may provide a basis for explaining the discrepancy in the literature concerning the effects of thrombin on platelet respiration.

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Oxygen consumption of human blood platelets. I. Effect of thrombin

Abstract

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