@inbook{38d2f23911414d729cac5080ba25e102,
title = "Optical Control of Genome Editing by Photoactivatable Cas9",
abstract = "The CRISPR-Cas9 system offers targeted genome manipulation with simplicity. Combining the CRISPR-Cas9 with optogenetics technology, we have engineered photoactivatable Cas9 to precisely control the genome sequence in a spatiotemporal manner. Here we provide a detailed protocol for optogenetic genome editing experiments using photoactivatable Cas9, including that for the generation of guide RNA vectors, light-mediated Cas9 activation, and quantification of genome editing efficiency in mammalian cells.",
keywords = "CRISPR-Cas9, Genome editing, Genomic PCR, Guide RNA, HDR, NHEJ, Optogenetics, T7E1 assay",
author = "Takahiro Otabe and Yuta Nihongaki and Moritoshi Sato",
note = "Funding Information: We would like to thank CREST grants (JPMJCR1653) from Japan Science and Technology Agency. This work was supported by a project grant from Kanagawa Institute of Industrial Science and Technology (KISTEC) to M.S. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1441-9_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "225--233",
booktitle = "Methods in Molecular Biology",
}