Optical clearing for luminal organ imaging with ultrahigh-resolution optical coherence tomography

Yanmei Liang, Wu Yuan, Jessica Mavadia-Shukla, Xingde Li

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The imaging depth of optical coherence tomography (OCT) in highly scattering biological tissues (such as luminal organs) is limited, particularly for OCT operating at shorter wavelength regions (such as around 800 nm). For the first time, the optical clearing effect of the mixture of liquid paraffin and glycerol on luminal organs was explored with ultrahigh-resolution spectral domain OCT at 800 nm. Ex vivo studies were performed on pig esophagus and bronchus, and Guinea pig esophagus with different volume ratios of the mixture. We found that the mixture of 40% liquid paraffin had the best optical clearing effect on esophageal tissues with a short effective time of 10 min, which means the clearing effect occurs about 10 min after the application of the clearing agent. In contrast, no obvious optical clearing effect was identified on bronchus tissues.

Original languageEnglish (US)
Article number081211
JournalJournal of biomedical optics
Issue number8
StatePublished - Aug 1 2016


  • luminal organs
  • optical clearing technique
  • optical coherence tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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