Opiate receptor: Autoradiographic localization in rat brain

C. B. Pert, M. J. Kuhar, S. H. Snyder

Research output: Contribution to journalArticlepeer-review

462 Scopus citations


Opiate receptor sites in rat brain can be labeled in vivo by [3H]diprenorphine, a potent opiate antagonist. Using techniques to minimize diffusion in fresh, frozen, unfixed brain, we have localized [3H]diprenorphine by autoradiography to visualize the distribution of opiate receptors. Silver grains indicative of the binding of labeled [3H]diprenorphine are discretely localized in numerous areas of the brain with very high densities in the locus coeruleus, the substantia gelatinosa of the spinal cord, and in clusters within the caudate putamen, amygdala, and parts of the periventricular gray matter.

Original languageEnglish (US)
Pages (from-to)3729-3733
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10
StatePublished - 1976

ASJC Scopus subject areas

  • General


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