TY - JOUR
T1 - Ontogeny of the O2-senstive pathway in medulla oblongata of postnatal rat
AU - White, L. D.
AU - Lawson, E. E.
AU - Millhorn, D. E.
N1 - Funding Information:
The authors express their appreciation to Luisa Brighton for her expert technical assistance. This work was supported by NIH Grants HL34919 (E.E.L.) and HL33831 and HD28948 (D.E.M.). L.D.W. is supported in part by Glaxo Pharmaceutical, Research Triangle Park, NC.
PY - 1994/10
Y1 - 1994/10
N2 - Fos protein, the product of the immediate early gene c-fos as a metabolic marker to map the O2 chemosensory pathway activated by hypoxia in the adult rat (Erickson and Millhorn, Brain Res. 567: 11-24, 1991). The current study provides evidence that the O2 chemoreceptor pathway develops during the first postnatal month. Rats at postnatal ages (P) 3, 7, 10, 14, 21, adn 28 days were exposed for 3 h to 21% (control) or 10% (hypoxia) O2. Pups were transcardially fixed, brain stems were froze, sectioned, then reacted Fos primary antibody, a secondary antibody, avidin-biotin peroxidase, then Ni-DAB as chromogen. Cells showing Fos-like immunoreactivity (Fos0LI) under control and hypoxic conditions were counted in the nucleus tractus solitarii (NTS) and the ventrolateral medulla (VLM). In both areas there was initially a low basal level of Fos-LI, a peak at P10 and a decline to P28. At all ages there was a significant increase in the number of Fos-LI cells in pups exposed to hypopxia. The high basal level of Fos expression at P10 and the high induced level at P14 may correlate with periods of terminal differentiation and maximum synaptogenesis, respectively.
AB - Fos protein, the product of the immediate early gene c-fos as a metabolic marker to map the O2 chemosensory pathway activated by hypoxia in the adult rat (Erickson and Millhorn, Brain Res. 567: 11-24, 1991). The current study provides evidence that the O2 chemoreceptor pathway develops during the first postnatal month. Rats at postnatal ages (P) 3, 7, 10, 14, 21, adn 28 days were exposed for 3 h to 21% (control) or 10% (hypoxia) O2. Pups were transcardially fixed, brain stems were froze, sectioned, then reacted Fos primary antibody, a secondary antibody, avidin-biotin peroxidase, then Ni-DAB as chromogen. Cells showing Fos-like immunoreactivity (Fos0LI) under control and hypoxic conditions were counted in the nucleus tractus solitarii (NTS) and the ventrolateral medulla (VLM). In both areas there was initially a low basal level of Fos-LI, a peak at P10 and a decline to P28. At all ages there was a significant increase in the number of Fos-LI cells in pups exposed to hypopxia. The high basal level of Fos expression at P10 and the high induced level at P14 may correlate with periods of terminal differentiation and maximum synaptogenesis, respectively.
KW - Chemosensitivity
KW - Contorl of breathgin
KW - Development
KW - Mammals
KW - Medulla
KW - O central pathway
KW - O chemoreceptor pathway
KW - O chemosensitivity
KW - nucleus tractus solitarii
KW - rat
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U2 - 10.1016/0034-5687(94)00055-7
DO - 10.1016/0034-5687(94)00055-7
M3 - Article
C2 - 7817045
AN - SCOPUS:0028168544
SN - 0034-5687
VL - 98
SP - 123
EP - 135
JO - Respiration Physiology
JF - Respiration Physiology
IS - 2
ER -