@inbook{7e07e4f27fd04c1d98ee9466ef636256,
title = "One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE)",
abstract = "This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.",
keywords = "Ammonium persulfate (APS), One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE), Polyacrylamide, Polyacrylamide gel electrophoresis, SDS-PAGE gel, Stacking gel, Tetramethylethylenediamine (TEMED)",
author = "Brunelle, {Julie L.} and Rachel Green",
year = "2014",
doi = "10.1016/B978-0-12-420119-4.00012-4",
language = "English (US)",
isbn = "9780124201194",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "151--159",
booktitle = "Laboratory Methods in Enzymology",
}