Ocular surface rehabilitation: Application of human amniotic membrane in high-risk penetrating keratoplasties

Pho Nguyen, Kelly Rue, Martin Heur, Samuel C. Yiu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Background: Human amniotic membrane is a versatile tool for management of ocular surface disorders. This study evaluates the effect of cryopreserved human amniotic membrane (hAM) on one-year survival of penetrating keratoplasties (PKP) in high-risk recipients. Method: This is a retrospective noncomparative cohort study of 58 consecutive eyes undergoing PKP with concurrent placement of a self-retained cryopreserved hAM (PROKERA®) at a tertiary care center from January 2009 to July 2010. Results: Mean patient age was 66.7. ±. 17.2. years and 30 (54%) were males. 51 eyes were pseudophakic and one aphakic. 27 eyes were glaucomatous; 24 had glaucoma drainage device and 2 had previous endocyclophotocoagulation. 12 patients had PKP for the first time and 46 had repeat PKP (average number of prior PKP = 1.63. ±. 1.1, range: 1-5).Risk factors for graft failure included repeat PKP (79.3%), corneal neovascularization (51.7%), preexisting glaucoma (46.6%), and presence of anterior synechiae (37.9%). Both First Transplant and Repeat Transplant groups had similar survival rates until 6. months after transplant (75% vs 74%, odds ratio = 1.06, p= 1.00). At 12. months, First Transplant group showed a better survival rate (67% vs 43%, odds ratio = 2.60, p= 0.20). Eyes with > 3 risk factors had a higher graft failure rate (odds ratio = 5.81, p= 0.003). Conclusion: Survey of the literature suggests that high-risk PKP with concurrent hAM placement demonstrate comparable graft survival. Presence of multiple risk factors is associated with poor survival.

Original languageEnglish (US)
Pages (from-to)198-202
Number of pages5
JournalSaudi Journal of Ophthalmology
Issue number3
StatePublished - Jul 1 2014


  • Amniotic membrane
  • Anti-angiogenesis
  • Graft rejection
  • Immunomodulation
  • Penetrating keratoplasty
  • ProKera

ASJC Scopus subject areas

  • Ophthalmology


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