Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds

Sarindr Bhumiratana, Warren L. Grayson, Andrea Castaneda, Danielle N. Rockwood, Eun S. Gil, David L. Kaplan, Gordana Vunjak-Novakovic

Research output: Contribution to journalArticlepeer-review

196 Scopus citations


We describe a composite hydroxyapatite (HA)-silk fibroin scaffold designed to induce and support the formation of mineralized bone matrix by human mesenchymal stem cells (hMSCs) in the absence of osteogenic growth factors. Porous three-dimensional silk scaffolds were extensively used in our previous work for bone tissue engineering and showed excellent biodegradability and biocompatibility. However, silk is not an osteogenic material and has a compressive stiffness significantly lower than that of native bone. In the present study, we explored the incorporation of silk sponge matrices with HA (bone mineral) micro-particles to generate highly osteogenic composite scaffolds capable of inducing the in vitro formation of tissue-engineered bone. Different amounts of HA were embedded in silk sponges at volume fractions of 0%, 1.6%, 3.1% and 4.6% to enhance the osteoconductive activity and mechanical properties of the scaffolds. The cultivation of hMSCs in the silk/HA composite scaffolds under perfusion conditions resulted in the formation of bone-like structures and an increase in the equilibrium Young's modulus (up to 4-fold or 8-fold over 5 or 10 weeks of cultivation, respectively) in a manner that correlated with the initial HA content. The enhancement in mechanical properties was associated with the development of the structural connectivity of engineered bone matrix. Collectively, the data suggest two mechanisms by which the incorporated HA enhanced the formation of tissue engineered bone: through osteoconductivity of the material leading to increased bone matrix production, and by providing nucleation sites for new mineral resulting in the connectivity of trabecular-like architecture.

Original languageEnglish (US)
Pages (from-to)2812-2820
Number of pages9
Issue number11
StatePublished - Apr 2011


  • Bone
  • Hydroxyapatite
  • Mineralization
  • Scaffold
  • Silk
  • Tissue engineering

ASJC Scopus subject areas

  • Mechanics of Materials
  • Ceramics and Composites
  • Bioengineering
  • Biophysics
  • Biomaterials


Dive into the research topics of 'Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds'. Together they form a unique fingerprint.

Cite this