Novel polyketides from genetic engineering (... and lessons we have learned from making them)

Leonard Katz, Jonathan Kennedy, Sarah C. Mutka, John R. Carney, Karen S. MacMillan, Sumati Murli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Knowledge of the domain organization of type I modular polyketide synthases (PKS) usually allows accurate prediction of the structure of the corresponding polyketide. Genetic engineering of such PKSs can be employed to produce novel compounds of predicted structure. Genetic engineering of the epothilone PKS has given unexpected results that have revealed subtleties of the biosynthesis not previously understood. In one instance, an unprecedented mechanism was uncovered.

Original languageEnglish (US)
Title of host publicationPolyketides Biosynthesis, Biological Activity, and Genetic Engineering
PublisherAmerican Chemical Society
Pages200-216
Number of pages17
ISBN (Print)0841239789, 9780841239784
DOIs
StatePublished - 2007
Externally publishedYes

Publication series

NameACS Symposium Series
Volume955
ISSN (Print)0097-6156

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering

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