@article{03153bd495f848b5b496193e783e0437,
title = "Noninvasive optical and nuclear imaging of Staphylococcus-specific infection with a human monoclonal antibody-based probe",
abstract = "Staphylococcus aureus infections are a major threat in healthcare, requiring adequate early-stage diagnosis and treatment. This calls for novel diagnostic tools that allow noninvasive in vivo detection of staphylococci. Here we performed a preclinical study to investigate a novel fully-human monoclonal antibody 1D9 that specifically targets the immunodominant staphylococcal antigen A (IsaA). We show that 1D9 binds invariantly to S. aureus cells and may further target other staphylococcal species. Importantly, using a human post-mortem implant model and an in vivo murine skin infection model, preclinical feasibility was demonstrated for 1D9 labeled with the near-infrared fluorophore IRDye800CW to be applied for direct optical imaging of in vivo S. aureus infections. Additionally, 89Zirconium-labeled 1D9 could be used for positron emission tomography imaging of an in vivo S. aureus thigh infection model. Our findings pave the way towards clinical implementation of targeted imaging of staphylococcal infections using the human monoclonal antibody 1D9.",
keywords = "IsaA, PET, Staphylococcus aureus, Zr, human monoclonal antibody, immunodominant staphylococcal antigen A",
author = "{Romero Pastrana}, Francisco and Thompson, {John M.} and Marjolein Heuker and Hedzer Hoekstra and Dillen, {Carly A.} and Ortines, {Roger V.} and Ashbaugh, {Alyssa G.} and Pickett, {Julie E.} and Linssen, {Matthijs D.} and Bernthal, {Nicholas M.} and Francis, {Kevin P.} and Girbe Buist and {van Oosten}, Marleen and {van Dam}, {Gooitzen M.} and Thorek, {Daniel L.J.} and Miller, {Lloyd S.} and {van Dijl}, {Jan Maarten}",
note = "Funding Information: Part of this research was supported by the Top Institute Pharma projects T4-213 and T4-502 (to J.M.v.D), and by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the U.S. National Institutes of Health grant numbers T32 AR067708 (J.M.T. and J.E.P.) and R01AR069502 (L.S.M.). F. Romero Pastrana received a scholarship from CONACyT (169643). The authors thank Trishla Sinha and Romano Schreuder for the isolation of staphylococcal strains. Funding Information: Part of this research was supported by the Top Institute Pharma projects T4-213 and T4-502 (to J.M.v.D), and by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the U.S. National Institutes of Health grant numbers T32 AR067708 (J.M.T. and J.E.P.) and R01AR069502 (L.S.M.). F. Romero Pastrana received a scholarship from CONACyT (169643). Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = jan,
day = "1",
doi = "10.1080/21505594.2017.1403004",
language = "English (US)",
volume = "9",
pages = "262--272",
journal = "Virulence",
issn = "2150-5594",
publisher = "Landes Bioscience",
number = "1",
}