Nonhuman sialic acid Neu5Gc is very low in human embryonic stem cell-derived neural precursors differentiated with B27/N2 and noggin: Implications for transplantation

Igor O. Nasonkin; Vassilis E. Koliatsos

doi:10.1016/j.expneurol.2006.05.002

Nonhuman sialic acid Neu5Gc is very low in human embryonic stem cell-derived neural precursors differentiated with B27/N2 and noggin: Implications for transplantation

Igor O. Nasonkin, Vassilis E. Koliatsos

School of Medicine

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A concern recently has been raised that human embryonic stem cell (HESC) lines cultured with currently available methods may have limited clinical usefulness due to the immunogenicity of the nonhuman sialic acid Neu5Gc incorporated into their membranes during culturing. We find this concern has little relevance to neural differentiation protocols with B27/N2/noggin because of the gradual decline of Neu5Gc to less than 1% in differentiating cells upon switching to B27/N2 medium.

Original language	English (US)
Pages (from-to)	525-529
Number of pages	5
Journal	Experimental Neurology
Volume	201
Issue number	2
DOIs	https://doi.org/10.1016/j.expneurol.2006.05.002
State	Published - Oct 2006

Keywords

Human embryonic stem cells
Immunogenicity
Neu5Gc
Neural differentiation
Sialic
Transplantation

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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10.1016/j.expneurol.2006.05.002

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title = "Nonhuman sialic acid Neu5Gc is very low in human embryonic stem cell-derived neural precursors differentiated with B27/N2 and noggin: Implications for transplantation",

abstract = "A concern recently has been raised that human embryonic stem cell (HESC) lines cultured with currently available methods may have limited clinical usefulness due to the immunogenicity of the nonhuman sialic acid Neu5Gc incorporated into their membranes during culturing. We find this concern has little relevance to neural differentiation protocols with B27/N2/noggin because of the gradual decline of Neu5Gc to less than 1% in differentiating cells upon switching to B27/N2 medium.",

keywords = "Human embryonic stem cells, Immunogenicity, Neu5Gc, Neural differentiation, Sialic, Transplantation",

author = "Nasonkin, {Igor O.} and Koliatsos, {Vassilis E.}",

note = "Funding Information: This work was supported by NIH grant RO1 NS045140-03 and the Muscular Dystrophy Association grant (V.E.K). We thank Glen Hatfield for his skillful RNA handling and RT-PCR analysis. We also thank Drs. Sulabha Argade and Anup Datta (UCSD Glycobiology Research and Training Center) for sialic acid analysis. ",

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AU - Nasonkin, Igor O.

AU - Koliatsos, Vassilis E.

N1 - Funding Information: This work was supported by NIH grant RO1 NS045140-03 and the Muscular Dystrophy Association grant (V.E.K). We thank Glen Hatfield for his skillful RNA handling and RT-PCR analysis. We also thank Drs. Sulabha Argade and Anup Datta (UCSD Glycobiology Research and Training Center) for sialic acid analysis.

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N2 - A concern recently has been raised that human embryonic stem cell (HESC) lines cultured with currently available methods may have limited clinical usefulness due to the immunogenicity of the nonhuman sialic acid Neu5Gc incorporated into their membranes during culturing. We find this concern has little relevance to neural differentiation protocols with B27/N2/noggin because of the gradual decline of Neu5Gc to less than 1% in differentiating cells upon switching to B27/N2 medium.

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KW - Sialic

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Nonhuman sialic acid Neu5Gc is very low in human embryonic stem cell-derived neural precursors differentiated with B27/N2 and noggin: Implications for transplantation

Abstract

Keywords

ASJC Scopus subject areas

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