Non-Viral methods for generating Integration-Free, induced Pluripotent stem cells

Xiao Yue Deng, Hu Wang, Tao Wang, Xian Tao Fang, Li Li Zou, Zhi Ying Li, Chang Bai Liu

Research output: Contribution to journalArticlepeer-review


Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.

Original languageEnglish (US)
Pages (from-to)153-158
Number of pages6
JournalCurrent Stem Cell Research and Therapy
Issue number2
StatePublished - Jan 1 2015
Externally publishedYes


  • DNA integration-free
  • Induced pluripotent stem cells
  • Non-viral methods

ASJC Scopus subject areas

  • Medicine (miscellaneous)


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