TY - JOUR
T1 - Non-Viral methods for generating Integration-Free, induced Pluripotent stem cells
AU - Deng, Xiao Yue
AU - Wang, Hu
AU - Wang, Tao
AU - Fang, Xian Tao
AU - Zou, Li Li
AU - Li, Zhi Ying
AU - Liu, Chang Bai
N1 - Publisher Copyright:
© 2015 Bentham Science Publishers.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.
AB - Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.
KW - DNA integration-free
KW - Induced pluripotent stem cells
KW - Non-viral methods
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U2 - 10.2174/1574888X09666140923101914
DO - 10.2174/1574888X09666140923101914
M3 - Article
C2 - 25248676
AN - SCOPUS:84926358786
SN - 1574-888X
VL - 10
SP - 153
EP - 158
JO - Current Stem Cell Research and Therapy
JF - Current Stem Cell Research and Therapy
IS - 2
ER -