@article{b8e6c079dbda4ad4bfdcea00d463ebf1,
title = "NMR studies of glucose and alanine utilization and maternal-embryonic nutrient transfer in the smooth dogfish, Mustelus canis",
abstract = "Captive dogfish, Mustelus canis, were injected with saline containing 13C-enriched alanine or glucose and permitted to metabolize labeled substrates. Blood plasma and other body fluids were analyzed using high field 13C nuclear magnetic resonance (NMR) spectroscopy. Both injected glucose and alanine are converted to lactate with alanine conversion more rapid than that of glucose. 13C-enriched alanine and lactate appear in intracapsular fluids after injection of 13C-enriched alanine into the maternal blood vascular system. Studies involving the injection of 13C-enriched nutrients into the maternal circulation can yield information on metabolite utilization and embryonic nutrition in viviparous matrotrophs.",
keywords = "Alanine, Dogfish, Gestation, Glucose, Metabolism, Mustelus, NMR, Shark, Spectroscopy, Viviparity",
author = "Graham, {Charles R.} and Calhoun Bond and Chacko, {V. P.} and Julian Lombardi",
note = "Funding Information: Aclinou,ledgentenrs-We wish to thank Dr Robert L. Board for providing seawater facilities and Martin Connaughton for feeding the sharks and maintaining the holding tanks at the College of Marine Sciencies of the University 01 Delaware in Lewes. Delaware. Specimen procurement was made possible through the advice and tireless efforts of Captains Steve Beyer and Don Churchill of the R.V. Skimmer. which is administered by the College of Marine Sciencies of the University of Delaware. Five specimens were generously provided by the National Aquarium in Baltimore. We also express our gratitude to Dr William Hettler for providing seawater facilities and laboratory space at the NOAA Southwest Fisheries Lab in Beaufort, North Carolina. Specimen procurement in Beaufort was made possible through the advice of Dr Frank Schwartz and through the valuable assistance of the captain and crew of the R. V. Cupricorn. which is administered by the UNC Institute of Marine Sciences. Morehead City, North Carolina. We also wish to acknowledge the-assistance of Tamarah Files. Elizabeth Laban. Valerie Comnaretta. and Brenda Abrigo in various aspects of the study. Special thanks to Randy Hofmann for his help in capturing and working with the Delaware specimens. Support for this research was provided by Loyola College faculty development funds to C.R.G. and the UNCG Research Council. UNCG Summer Faculty Fellowships. and UNC Institute 01 Nutrition. and NSF Grant DCB-8711304 to J.L. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1995",
month = jun,
doi = "10.1016/0300-9629(95)00017-2",
language = "English (US)",
volume = "111",
pages = "199--207",
journal = "Comparative Biochemistry and Physiology -- Part A: Physiology",
issn = "0300-9629",
number = "2",
}