Nitroreductase-mediated cell ablation in transgenic zebrafish embryos.

Harshan Pisharath; Michael J. Parsons

doi:10.1007/978-1-60327-977-2_9

Nitroreductase-mediated cell ablation in transgenic zebrafish embryos.

Harshan Pisharath, Michael J. Parsons

School of Medicine

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.

Original language	English (US)
Pages (from-to)	133-143
Number of pages	11
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	546
DOIs	https://doi.org/10.1007/978-1-60327-977-2_9
State	Published - 2009

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-60327-977-2_9

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abstract = "Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.",

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AB - Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.

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Nitroreductase-mediated cell ablation in transgenic zebrafish embryos.

Abstract

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