TY - JOUR
T1 - Nitric oxide modulates human chorionic gonadotropin-induced ovulation in the rabbit
AU - Hesla, John S.
AU - Preutthipan, Sangchai
AU - Maguire, Michael P.
AU - Chang, Thomas S.K.
AU - Wallach, Edward E.
AU - Dharmarajan, Arunasalam M.
N1 - Funding Information:
Nitric oxide (NO), a gas with free radical chemical properties, is synthesized from the terminal guanidino nitrogen atom(s) of L-arginine by the enzyme NO Received May 6, 1996; revised and accepted September 26. 1996. * Supported by grants HD-19430 IE.E.W., A.M.D.) and HD-30137 (T.S.K.G.J from the National Institutes of Health, Bethesda, Maryland, and the Rockefeller Foundation, New York, New York (A.M.D.~. t First prize poster presentation at 49th Annual Meeting of The American Fertility Society (currently the American Society for Reproductive Medicine), Toronto, Ontario, October 9 to 14, 1993. $ Department of Gynecologya nd Obstetrics. § Reprint requests and present address: John S. Hesla, M.D., The Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 300, Englewood, Colorado 80110 (FAX: 303-788-8310).
PY - 1997
Y1 - 1997
N2 - Objective: To examine the potential role of the L-arginine:nitric oxide pathway in hCG-induced ovulation in the rabbit. Design: Randomized, controlled animal study. Setting: University research laboratory. Intervention(s): Nitric oxide synthase, the enzyme that produces nitric oxide (NO), was immunohistochemically localized in the ovary. N(G)-nitro-L- arginine methyl ester (L-NAME), an analogue of L-arginine, which inhibits the enzyme NO synthase, and the inactive D-enantiomer were administered in vivo and/or in vitro via an isolated, perfused ovary preparation during the periovulatory period. Main Outcome Measure(s): Rate of follicular rupture/ovulatory efficiency). Result(s): Immunohistochemical staining for NO synthase was localized specifically to the granulosa cell layer of the follicle and the endothelium and adventitia of ovarian blood vessels. In vivo administration of L-NAME significantly reduced the percentage of large follicles that ovulated in response to hCG (treated 24.6%, control 68.1%). Similarly, exposure of the in vitro-perfused ovary to L-NAME significantly reduced follicular rupture (treated 32.8%, control 64.2%). In contrast, addition of an equimolar concentration of D-NAME to the perfusion medium had no significant effect on the rate of ovulation (treated 83.3%, control 61.3%). Conclusion(s): The stereospecific inhibition of follicular rupture by the arginine analogue suggests that NO production by the ovary is an important feature of the normal physiologic processes of the periovulatory period.
AB - Objective: To examine the potential role of the L-arginine:nitric oxide pathway in hCG-induced ovulation in the rabbit. Design: Randomized, controlled animal study. Setting: University research laboratory. Intervention(s): Nitric oxide synthase, the enzyme that produces nitric oxide (NO), was immunohistochemically localized in the ovary. N(G)-nitro-L- arginine methyl ester (L-NAME), an analogue of L-arginine, which inhibits the enzyme NO synthase, and the inactive D-enantiomer were administered in vivo and/or in vitro via an isolated, perfused ovary preparation during the periovulatory period. Main Outcome Measure(s): Rate of follicular rupture/ovulatory efficiency). Result(s): Immunohistochemical staining for NO synthase was localized specifically to the granulosa cell layer of the follicle and the endothelium and adventitia of ovarian blood vessels. In vivo administration of L-NAME significantly reduced the percentage of large follicles that ovulated in response to hCG (treated 24.6%, control 68.1%). Similarly, exposure of the in vitro-perfused ovary to L-NAME significantly reduced follicular rupture (treated 32.8%, control 64.2%). In contrast, addition of an equimolar concentration of D-NAME to the perfusion medium had no significant effect on the rate of ovulation (treated 83.3%, control 61.3%). Conclusion(s): The stereospecific inhibition of follicular rupture by the arginine analogue suggests that NO production by the ovary is an important feature of the normal physiologic processes of the periovulatory period.
KW - Nitric oxide
KW - ovary
KW - ovulation
KW - rabbit
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U2 - 10.1016/S0015-0282(97)80084-2
DO - 10.1016/S0015-0282(97)80084-2
M3 - Article
C2 - 9091345
AN - SCOPUS:0031028698
SN - 0015-0282
VL - 67
SP - 548
EP - 552
JO - Fertility and sterility
JF - Fertility and sterility
IS - 3
ER -