TY - JOUR
T1 - New HLA-A, -B, and -C locus-specific primers for PCR amplification from cDNA
T2 - Application in clinical immunology
AU - Bettinotti, Maria P.
AU - Hadzikadic, Lejla
AU - Ruppe, Erin
AU - Dhillon, Gitika
AU - Stroncek, David S.
AU - Marincola, Francesco M.
PY - 2003/8
Y1 - 2003/8
N2 - The individual cellular immune response to intracellular antigens is modeled by the highly polymorphic major histocompatibility complex (HLA) class I molecules. The epitopes presented and the T cell repertoire that recognizes them depend on the HLA constitution of the individual. Therefore, to monitor and to modify an individual's HLA class I-driven cellular immune response, it is necessary to know the HLA class I alleles of the person and the possible epitopes of the target antigen presented by those alleles. In particular, this is necessary in order to design peptide-based vaccines and immune therapies for the treatment of diseases caused by viruses, intracellular parasites or cancer, and to monitor the immune response during those treatments. We describe a new set of HLA-A, -B, and -C locus-specific primers for the polymerase chain reaction (PCR) amplification of the whole coding sequence of these genes from complementary DNA (cDNA). We describe their use for typing and for the production of a library of recombinant HLA class I genes. We discuss two downstream applications of this gene collection: production of soluble HLA molecules and discovery of new epitopes. Crown
AB - The individual cellular immune response to intracellular antigens is modeled by the highly polymorphic major histocompatibility complex (HLA) class I molecules. The epitopes presented and the T cell repertoire that recognizes them depend on the HLA constitution of the individual. Therefore, to monitor and to modify an individual's HLA class I-driven cellular immune response, it is necessary to know the HLA class I alleles of the person and the possible epitopes of the target antigen presented by those alleles. In particular, this is necessary in order to design peptide-based vaccines and immune therapies for the treatment of diseases caused by viruses, intracellular parasites or cancer, and to monitor the immune response during those treatments. We describe a new set of HLA-A, -B, and -C locus-specific primers for the polymerase chain reaction (PCR) amplification of the whole coding sequence of these genes from complementary DNA (cDNA). We describe their use for typing and for the production of a library of recombinant HLA class I genes. We discuss two downstream applications of this gene collection: production of soluble HLA molecules and discovery of new epitopes. Crown
KW - Complementary deoxyribonucleic acid
KW - Human leukocyte antigen
KW - Polymerase chain reaction
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U2 - 10.1016/S0022-1759(03)00233-3
DO - 10.1016/S0022-1759(03)00233-3
M3 - Article
C2 - 12969555
AN - SCOPUS:0142061085
SN - 0022-1759
VL - 279
SP - 143
EP - 148
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -