Neutrophil extracellular trap formation in anti-neutrophil cytoplasmic antibody-associated and large-vessel vasculitis

Despina Michailidou; Runa Kuley; Ting Wang; Payton Hermanson; Peter C. Grayson; David Cuthbertson; Nader A. Khalidi; Curry L. Koening; Carol A. Langford; Carol A. McAlear; Larry W. Moreland; Christian Pagnoux; Philip Seo; Ulrich Specks; Antoine G. Sreih; Kenneth J. Warrington; Paul A. Monach; Peter A. Merkel; Christian Lood

doi:10.1016/j.clim.2023.109274

Neutrophil extracellular trap formation in anti-neutrophil cytoplasmic antibody-associated and large-vessel vasculitis

Despina Michailidou, Runa Kuley, Ting Wang, Payton Hermanson, Peter C. Grayson, David Cuthbertson, Nader A. Khalidi, Curry L. Koening, Carol A. Langford, Carol A. McAlear, Larry W. Moreland, Christian Pagnoux, Philip Seo, Ulrich Specks, Antoine G. Sreih, Kenneth J. Warrington, Paul A. Monach, Peter A. Merkel, Christian Lood

Research output: Contribution to journal › Article › peer-review

Abstract

Levels of neutrophil extracellular traps (NETs) were measured in plasma of healthy controls (HC, n = 30) and patients with granulomatosis with polyangiitis (GPA, n = 123), microscopic polyangiitis (MPA, n = 61), Takayasu's arteritis (TAK, n = 58), and giant cell arteritis (GCA, n = 68), at times of remission or activity and correlated with levels of the platelet-derived thrombospondin-1 (TSP-1). Levels of NETs were elevated during active disease in patients with GPA (p < 0.0001), MPA (p = 0.0038), TAK (p < 0.0001), and GCA (p < 0.0001), and in remission for GPA, p < 0.0001, MPA, p = 0.005, TAK, p = 0.03, and GCA, p = 0.0009. All cohorts demonstrated impaired NET degradation. Patients with GPA (p = 0.0045) and MPA (p = 0.005) had anti-NET IgG antibodies. Patients with TAK had anti-histone antibodies (p < 0.01), correlating with presence of NETs. Levels of TSP-1 were increased in all patients with vasculitis, and associated with NET formation. NET formation is a common process in vasculitides. Targeting NET formation or degradation could be potential therapeutic approaches for vasculitides.

Original language	English (US)
Article number	109274
Journal	Clinical Immunology
Volume	249
DOIs	https://doi.org/10.1016/j.clim.2023.109274
State	Published - Apr 2023
Externally published	Yes

Keywords

Anti-neutrophil cytoplasmic antibody associated vasculitis
Histone
Large vessel vasculitis
Neutrophil extracellular traps
Neutrophils
Platelet activation
Thrombospondin-1

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.clim.2023.109274

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Michailidou, D., Kuley, R., Wang, T., Hermanson, P., Grayson, P. C., Cuthbertson, D., Khalidi, N. A., Koening, C. L., Langford, C. A., McAlear, C. A., Moreland, L. W., Pagnoux, C., Seo, P., Specks, U., Sreih, A. G., Warrington, K. J., Monach, P. A., Merkel, P. A., & Lood, C. (2023). Neutrophil extracellular trap formation in anti-neutrophil cytoplasmic antibody-associated and large-vessel vasculitis. Clinical Immunology, 249, Article 109274. https://doi.org/10.1016/j.clim.2023.109274

Michailidou, D, Kuley, R, Wang, T, Hermanson, P, Grayson, PC, Cuthbertson, D, Khalidi, NA, Koening, CL, Langford, CA, McAlear, CA, Moreland, LW, Pagnoux, C, Seo, P, Specks, U, Sreih, AG, Warrington, KJ, Monach, PA, Merkel, PA & Lood, C 2023, 'Neutrophil extracellular trap formation in anti-neutrophil cytoplasmic antibody-associated and large-vessel vasculitis', Clinical Immunology, vol. 249, 109274. https://doi.org/10.1016/j.clim.2023.109274

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title = "Neutrophil extracellular trap formation in anti-neutrophil cytoplasmic antibody-associated and large-vessel vasculitis",

abstract = "Levels of neutrophil extracellular traps (NETs) were measured in plasma of healthy controls (HC, n = 30) and patients with granulomatosis with polyangiitis (GPA, n = 123), microscopic polyangiitis (MPA, n = 61), Takayasu's arteritis (TAK, n = 58), and giant cell arteritis (GCA, n = 68), at times of remission or activity and correlated with levels of the platelet-derived thrombospondin-1 (TSP-1). Levels of NETs were elevated during active disease in patients with GPA (p < 0.0001), MPA (p = 0.0038), TAK (p < 0.0001), and GCA (p < 0.0001), and in remission for GPA, p < 0.0001, MPA, p = 0.005, TAK, p = 0.03, and GCA, p = 0.0009. All cohorts demonstrated impaired NET degradation. Patients with GPA (p = 0.0045) and MPA (p = 0.005) had anti-NET IgG antibodies. Patients with TAK had anti-histone antibodies (p < 0.01), correlating with presence of NETs. Levels of TSP-1 were increased in all patients with vasculitis, and associated with NET formation. NET formation is a common process in vasculitides. Targeting NET formation or degradation could be potential therapeutic approaches for vasculitides.",

keywords = "Anti-neutrophil cytoplasmic antibody associated vasculitis, Histone, Large vessel vasculitis, Neutrophil extracellular traps, Neutrophils, Platelet activation, Thrombospondin-1",

author = "Despina Michailidou and Runa Kuley and Ting Wang and Payton Hermanson and Grayson, {Peter C.} and David Cuthbertson and Khalidi, {Nader A.} and Koening, {Curry L.} and Langford, {Carol A.} and McAlear, {Carol A.} and Moreland, {Larry W.} and Christian Pagnoux and Philip Seo and Ulrich Specks and Sreih, {Antoine G.} and Warrington, {Kenneth J.} and Monach, {Paul A.} and Merkel, {Peter A.} and Christian Lood",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier Inc.",

year = "2023",

month = apr,

doi = "10.1016/j.clim.2023.109274",

language = "English (US)",

volume = "249",

journal = "Clinical Immunology",

issn = "1521-6616",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Neutrophil extracellular trap formation in anti-neutrophil cytoplasmic antibody-associated and large-vessel vasculitis

AU - Michailidou, Despina

AU - Kuley, Runa

AU - Wang, Ting

AU - Hermanson, Payton

AU - Grayson, Peter C.

AU - Cuthbertson, David

AU - Khalidi, Nader A.

AU - Koening, Curry L.

AU - Langford, Carol A.

AU - McAlear, Carol A.

AU - Moreland, Larry W.

AU - Pagnoux, Christian

AU - Seo, Philip

AU - Specks, Ulrich

AU - Sreih, Antoine G.

AU - Warrington, Kenneth J.

AU - Monach, Paul A.

AU - Merkel, Peter A.

AU - Lood, Christian

PY - 2023/4

Y1 - 2023/4

N2 - Levels of neutrophil extracellular traps (NETs) were measured in plasma of healthy controls (HC, n = 30) and patients with granulomatosis with polyangiitis (GPA, n = 123), microscopic polyangiitis (MPA, n = 61), Takayasu's arteritis (TAK, n = 58), and giant cell arteritis (GCA, n = 68), at times of remission or activity and correlated with levels of the platelet-derived thrombospondin-1 (TSP-1). Levels of NETs were elevated during active disease in patients with GPA (p < 0.0001), MPA (p = 0.0038), TAK (p < 0.0001), and GCA (p < 0.0001), and in remission for GPA, p < 0.0001, MPA, p = 0.005, TAK, p = 0.03, and GCA, p = 0.0009. All cohorts demonstrated impaired NET degradation. Patients with GPA (p = 0.0045) and MPA (p = 0.005) had anti-NET IgG antibodies. Patients with TAK had anti-histone antibodies (p < 0.01), correlating with presence of NETs. Levels of TSP-1 were increased in all patients with vasculitis, and associated with NET formation. NET formation is a common process in vasculitides. Targeting NET formation or degradation could be potential therapeutic approaches for vasculitides.

AB - Levels of neutrophil extracellular traps (NETs) were measured in plasma of healthy controls (HC, n = 30) and patients with granulomatosis with polyangiitis (GPA, n = 123), microscopic polyangiitis (MPA, n = 61), Takayasu's arteritis (TAK, n = 58), and giant cell arteritis (GCA, n = 68), at times of remission or activity and correlated with levels of the platelet-derived thrombospondin-1 (TSP-1). Levels of NETs were elevated during active disease in patients with GPA (p < 0.0001), MPA (p = 0.0038), TAK (p < 0.0001), and GCA (p < 0.0001), and in remission for GPA, p < 0.0001, MPA, p = 0.005, TAK, p = 0.03, and GCA, p = 0.0009. All cohorts demonstrated impaired NET degradation. Patients with GPA (p = 0.0045) and MPA (p = 0.005) had anti-NET IgG antibodies. Patients with TAK had anti-histone antibodies (p < 0.01), correlating with presence of NETs. Levels of TSP-1 were increased in all patients with vasculitis, and associated with NET formation. NET formation is a common process in vasculitides. Targeting NET formation or degradation could be potential therapeutic approaches for vasculitides.

KW - Anti-neutrophil cytoplasmic antibody associated vasculitis

KW - Histone

KW - Large vessel vasculitis

KW - Neutrophil extracellular traps

KW - Neutrophils

KW - Platelet activation

KW - Thrombospondin-1

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U2 - 10.1016/j.clim.2023.109274

DO - 10.1016/j.clim.2023.109274

M3 - Article

C2 - 36878421

AN - SCOPUS:85150926332

SN - 1521-6616

VL - 249

JO - Clinical Immunology

JF - Clinical Immunology

M1 - 109274

ER -

Neutrophil extracellular trap formation in anti-neutrophil cytoplasmic antibody-associated and large-vessel vasculitis

Abstract

Keywords

ASJC Scopus subject areas

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