Neuronal gene delivery with replication-competent non-cytopathic sindbis virus

Yien Che Tsai, George Oyler, Nitish Thakor

Research output: Contribution to journalConference articlepeer-review


Sindbis Virus (SV) is an effective vector for delivering genes to neurons in-vivo. Current SV vectors induce apoptosis in host cells, limiting their usefulness. We have engineered a non-cytopathic mutation into 4 different strains of SV capable of expression in mice of different ages. The resulting NCP SV vectors allow prolonged high level expression of heterologous proteins in-vitro and in-vivo. In general, the ability to introduce new properties into the SV vector by independent mutations that affect cytopathology, age range of host, and temperature inducibility suggests great adaptability of the SV system as a gene delivery vector.

Original languageEnglish (US)
Pages (from-to)S-125
JournalAnnals of biomedical engineering
Issue numberSUPPL. 1
StatePublished - 2000
Event2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA
Duration: Oct 12 2000Oct 14 2000

ASJC Scopus subject areas

  • Biomedical Engineering


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