Nerve growth factor regulates VR-1 mRNA levels in cultures of adult dorsal root ganglion neurons

John Winston; Hiroki Toma; Mohan Shenoy; Pankaj Jay Pasricha

doi:10.1016/S0304-3959(00)00370-5

Nerve growth factor regulates VR-1 mRNA levels in cultures of adult dorsal root ganglion neurons

John Winston, Hiroki Toma, Mohan Shenoy, Pankaj Jay Pasricha

Research output: Contribution to journal › Article › peer-review

163 Scopus citations

Abstract

Nerve growth factor (NGF) regulates the nociceptive properties including sensitivity to capsaicin of a subset of dorsal root ganglion neurons, which express the high-affinity NGF receptor, trkA. Capsaicin sensitivity co-localizes with the expression of a cloned capsaicin receptor, vanilloid receptor type 1 (VR-1), which displays properties similar to the native capsaicin response. To determine whether VR-1 mRNA levels are regulated by NGF, VR-1 mRNA levels and the ability to respond to capsaicin by release of the neuropeptide calcitonin gene related peptide (CGRP) were measured as a function of NGF concentration in cultures of adult dorsal root ganglion neurons. NGF treatment increased both VR-1 mRNA expression and capsaicin evoked release of CGRP. These effects were inhibited by treatment with the trkA inhibitor k252a.

Original language	English (US)
Pages (from-to)	181-186
Number of pages	6
Journal	Pain
Volume	89
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3959(00)00370-5
State	Published - 2001
Externally published	Yes

Keywords

Calcitonin gene related peptide release
Dorsal root ganglion
Nerve growth factor
Vanilloid receptor

ASJC Scopus subject areas

Neurology
Clinical Neurology
Anesthesiology and Pain Medicine

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10.1016/S0304-3959(00)00370-5

Cite this

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title = "Nerve growth factor regulates VR-1 mRNA levels in cultures of adult dorsal root ganglion neurons",

abstract = "Nerve growth factor (NGF) regulates the nociceptive properties including sensitivity to capsaicin of a subset of dorsal root ganglion neurons, which express the high-affinity NGF receptor, trkA. Capsaicin sensitivity co-localizes with the expression of a cloned capsaicin receptor, vanilloid receptor type 1 (VR-1), which displays properties similar to the native capsaicin response. To determine whether VR-1 mRNA levels are regulated by NGF, VR-1 mRNA levels and the ability to respond to capsaicin by release of the neuropeptide calcitonin gene related peptide (CGRP) were measured as a function of NGF concentration in cultures of adult dorsal root ganglion neurons. NGF treatment increased both VR-1 mRNA expression and capsaicin evoked release of CGRP. These effects were inhibited by treatment with the trkA inhibitor k252a.",

keywords = "Calcitonin gene related peptide release, Dorsal root ganglion, Nerve growth factor, Vanilloid receptor",

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AU - Winston, John

AU - Toma, Hiroki

AU - Shenoy, Mohan

AU - Pasricha, Pankaj Jay

PY - 2001

Y1 - 2001

N2 - Nerve growth factor (NGF) regulates the nociceptive properties including sensitivity to capsaicin of a subset of dorsal root ganglion neurons, which express the high-affinity NGF receptor, trkA. Capsaicin sensitivity co-localizes with the expression of a cloned capsaicin receptor, vanilloid receptor type 1 (VR-1), which displays properties similar to the native capsaicin response. To determine whether VR-1 mRNA levels are regulated by NGF, VR-1 mRNA levels and the ability to respond to capsaicin by release of the neuropeptide calcitonin gene related peptide (CGRP) were measured as a function of NGF concentration in cultures of adult dorsal root ganglion neurons. NGF treatment increased both VR-1 mRNA expression and capsaicin evoked release of CGRP. These effects were inhibited by treatment with the trkA inhibitor k252a.

AB - Nerve growth factor (NGF) regulates the nociceptive properties including sensitivity to capsaicin of a subset of dorsal root ganglion neurons, which express the high-affinity NGF receptor, trkA. Capsaicin sensitivity co-localizes with the expression of a cloned capsaicin receptor, vanilloid receptor type 1 (VR-1), which displays properties similar to the native capsaicin response. To determine whether VR-1 mRNA levels are regulated by NGF, VR-1 mRNA levels and the ability to respond to capsaicin by release of the neuropeptide calcitonin gene related peptide (CGRP) were measured as a function of NGF concentration in cultures of adult dorsal root ganglion neurons. NGF treatment increased both VR-1 mRNA expression and capsaicin evoked release of CGRP. These effects were inhibited by treatment with the trkA inhibitor k252a.

KW - Calcitonin gene related peptide release

KW - Dorsal root ganglion

KW - Nerve growth factor

KW - Vanilloid receptor

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Nerve growth factor regulates VR-1 mRNA levels in cultures of adult dorsal root ganglion neurons

Abstract

Keywords

ASJC Scopus subject areas

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