Nanoparticle fullerol alleviates radiculopathy via NLRP3 inflammasome and neuropeptides

Li Jin; Mengmeng Ding; Azra Oklopcic; Bayan Aghdasi; Li Xiao; Ziyi Li; Vesna Jevtovic-Todorovic; Xudong Li

doi:10.1016/j.nano.2017.03.015

Nanoparticle fullerol alleviates radiculopathy via NLRP3 inflammasome and neuropeptides

Li Jin, Mengmeng Ding, Azra Oklopcic, Bayan Aghdasi, Li Xiao, Ziyi Li, Vesna Jevtovic-Todorovic, Xudong Li

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The present study aimed to evaluate the analgesic effect of the antioxidant nanoparticle fullerol in a mouse radiculopathy and a dorsal root ganglion (DRG) culture models. Intervertebral disk degeneration causes significant hyperalgesia and nerve inflammation. Pain sensitization and inflammatory reaction were counteracted by fullerol when disk material was bathed in 10 or 100 μM of fullerol prior to implantation. Immunohistochemistry showed similar massive IBA1 positive macrophage infiltration surrounding implanted disk material among groups, but IL-1β and IL-6 expression was decreased in the fullerol treated group. In the DRG explant culture, after treatment with TNF-α, the expression of IL-1β, NLRP3, and caspase 1 was significantly increased but this was reversed by the addition of fullerol. In addition, fullerol also decreased the expression of substance P and CGRP in the cultured DRGs. Nanoparticle fullerol effectively counteracts pain sensitization and the inflammatory cascade caused by disk degeneration.

Original language	English (US)
Pages (from-to)	2049-2059
Number of pages	11
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	13
Issue number	6
DOIs	https://doi.org/10.1016/j.nano.2017.03.015
State	Published - Aug 2017
Externally published	Yes

Keywords

DRG
Fullerene
Hyperalgesia
Inflammasome
Low back pain
Radiculopathy

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Materials Science(all)
Pharmaceutical Science

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10.1016/j.nano.2017.03.015

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title = "Nanoparticle fullerol alleviates radiculopathy via NLRP3 inflammasome and neuropeptides",

abstract = "The present study aimed to evaluate the analgesic effect of the antioxidant nanoparticle fullerol in a mouse radiculopathy and a dorsal root ganglion (DRG) culture models. Intervertebral disk degeneration causes significant hyperalgesia and nerve inflammation. Pain sensitization and inflammatory reaction were counteracted by fullerol when disk material was bathed in 10 or 100 μM of fullerol prior to implantation. Immunohistochemistry showed similar massive IBA1 positive macrophage infiltration surrounding implanted disk material among groups, but IL-1β and IL-6 expression was decreased in the fullerol treated group. In the DRG explant culture, after treatment with TNF-α, the expression of IL-1β, NLRP3, and caspase 1 was significantly increased but this was reversed by the addition of fullerol. In addition, fullerol also decreased the expression of substance P and CGRP in the cultured DRGs. Nanoparticle fullerol effectively counteracts pain sensitization and the inflammatory cascade caused by disk degeneration.",

keywords = "DRG, Fullerene, Hyperalgesia, Inflammasome, Low back pain, Radiculopathy",

author = "Li Jin and Mengmeng Ding and Azra Oklopcic and Bayan Aghdasi and Li Xiao and Ziyi Li and Vesna Jevtovic-Todorovic and Xudong Li",

note = "Funding Information: Funding source: This work was supported by the National Institutes of Health(RO1AR064792 and R21AR057512) and North American Spine Society to XL. Funding sources had no involvement in the study. Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

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AU - Jin, Li

AU - Ding, Mengmeng

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AU - Aghdasi, Bayan

AU - Xiao, Li

AU - Li, Ziyi

AU - Jevtovic-Todorovic, Vesna

AU - Li, Xudong

N1 - Funding Information: Funding source: This work was supported by the National Institutes of Health(RO1AR064792 and R21AR057512) and North American Spine Society to XL. Funding sources had no involvement in the study. Publisher Copyright: © 2017 Elsevier Inc.

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N2 - The present study aimed to evaluate the analgesic effect of the antioxidant nanoparticle fullerol in a mouse radiculopathy and a dorsal root ganglion (DRG) culture models. Intervertebral disk degeneration causes significant hyperalgesia and nerve inflammation. Pain sensitization and inflammatory reaction were counteracted by fullerol when disk material was bathed in 10 or 100 μM of fullerol prior to implantation. Immunohistochemistry showed similar massive IBA1 positive macrophage infiltration surrounding implanted disk material among groups, but IL-1β and IL-6 expression was decreased in the fullerol treated group. In the DRG explant culture, after treatment with TNF-α, the expression of IL-1β, NLRP3, and caspase 1 was significantly increased but this was reversed by the addition of fullerol. In addition, fullerol also decreased the expression of substance P and CGRP in the cultured DRGs. Nanoparticle fullerol effectively counteracts pain sensitization and the inflammatory cascade caused by disk degeneration.

AB - The present study aimed to evaluate the analgesic effect of the antioxidant nanoparticle fullerol in a mouse radiculopathy and a dorsal root ganglion (DRG) culture models. Intervertebral disk degeneration causes significant hyperalgesia and nerve inflammation. Pain sensitization and inflammatory reaction were counteracted by fullerol when disk material was bathed in 10 or 100 μM of fullerol prior to implantation. Immunohistochemistry showed similar massive IBA1 positive macrophage infiltration surrounding implanted disk material among groups, but IL-1β and IL-6 expression was decreased in the fullerol treated group. In the DRG explant culture, after treatment with TNF-α, the expression of IL-1β, NLRP3, and caspase 1 was significantly increased but this was reversed by the addition of fullerol. In addition, fullerol also decreased the expression of substance P and CGRP in the cultured DRGs. Nanoparticle fullerol effectively counteracts pain sensitization and the inflammatory cascade caused by disk degeneration.

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Nanoparticle fullerol alleviates radiculopathy via NLRP3 inflammasome and neuropeptides

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