Nanofibrous nerve conduit-enhanced peripheral nerve regeneration

Xu Jiang, Ruifa Mi, Ahmet Hoke, Sing Yian Chew

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


Fibre structures represent a potential class of materials for the formation of synthetic nerve conduits due to their biomimicking architecture. Although the advantages of fibres in enhancing nerve regeneration have been demonstrated, in vivo evaluation of fibre size effect on nerve regeneration remains limited. In this study, we analyzed the effects of fibre diameter of electrospun conduits on peripheral nerve regeneration across a 15-mm critical defect gap in a rat sciatic nerve injury model. By using an electrospinning technique, fibrous conduits comprised of aligned electrospun poly (ε-caprolactone) (PCL) microfibers (981±83 nm, Microfiber) or nanofibers (251±32 nm, Nanofiber) were obtained. At three months post implantation, axons regenerated across the defect gap in all animals that received fibrous conduits. In contrast, complete nerve regeneration was not observed in the control group that received empty, non-porous PCL film conduits (Film). Nanofiber conduits resulted in significantly higher total number of myelinated axons and thicker myelin sheaths compared to Microfiber and Film conduits. Retrograde labeling revealed a significant increase in number of regenerated dorsal root ganglion sensory neurons in the presence of Nanofiber conduits (1.93 ± 0.71 × 103 vs. 0.98 ± 0.30 × 103 in Microfiber, p<0.01). In addition, the compound muscle action potential (CMAP) amplitudes were higher and distal motor latency values were lower in the Nanofiber conduit group compared to the Microfiber group. This study demonstrated the impact of fibre size on peripheral nerve regeneration. These results could provide useful insights for future nerve guide designs.

Original languageEnglish (US)
Pages (from-to)377-385
Number of pages9
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number5
StatePublished - May 2014


  • Contact guidance
  • Critical defect gap
  • Electrospinning
  • Nanofibers
  • Neural tissue engineering
  • Sciatic nerve regeneration

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


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