n-Sec1: A neural-specific syntaxin-binding protein

Jonathan Pevsner, Shu Chan Hsu, Richard H. Scheller

Research output: Contribution to journalArticlepeer-review

344 Scopus citations


We have identified n-Sec1, a rat brain homolog of the yeast Sec1p protein that participates in the constitutive secretory pathway between the Golgi apparatus and the plasma membrane. The rat brain cDNA is predicted to encode a 68-kDa protein with 65% amino acid identity to Drosophila rop, 59% identity to Caenorhabditis elegans unc-18, and 27% identity to Saccharomyces cerevisiae Sec1p. By RNA blot analysis, n-Sec1 mRNA expression is neural- specific. An anti-peptide antiserum directed against the n-Sec1 carboxyl terminus detects a 68-kDa protein in rat brain cytosol and membranes, but not in peripheral tissues. In the presence of syntaxin 1a, a plasma membrane protein implicated in synaptic vesicle docking, n-Sec1 becomes membrane- associated. n-Sec1 binds to syntaxin 1a, 2, and 3 fusion proteins coupled to agarose beads, but not to syntaxin 4 fusion protein or beads coupled to a variety of other proteins. These findings indicate that n-Sec1 is a neural- specific, syntaxin-binding protein that may participate in the regulation of synaptic vesicle docking and fusion.

Original languageEnglish (US)
Pages (from-to)1445-1449
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number4
StatePublished - Feb 15 1994
Externally publishedYes

ASJC Scopus subject areas

  • General


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