TY - JOUR
T1 - Mutation of the zinc-binding metalloprotease motif affects Bacteroides fragilis toxin activity but does not affect propeptide processing
AU - Franco-Mora, Augusto A
AU - Buckwold, Simy L.
AU - Shin, Jai W.
AU - Ascon, Miguel
AU - Sears, Cynthia L.
PY - 2005/8
Y1 - 2005/8
N2 - To evaluate the role of the zinc-binding metalloprotease in Bacteroides fragilis toxin (BFT) processing and activity, the zinc-binding consensus sequences (H348, E349, H352, G355, H358, and M366) were mutated by site-directed-mutagenesis. Our results indicated that single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro.
AB - To evaluate the role of the zinc-binding metalloprotease in Bacteroides fragilis toxin (BFT) processing and activity, the zinc-binding consensus sequences (H348, E349, H352, G355, H358, and M366) were mutated by site-directed-mutagenesis. Our results indicated that single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro.
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U2 - 10.1128/IAI.73.8.5273-5277.2005
DO - 10.1128/IAI.73.8.5273-5277.2005
M3 - Article
C2 - 16041055
AN - SCOPUS:23344446430
SN - 0019-9567
VL - 73
SP - 5273
EP - 5277
JO - Infection and immunity
JF - Infection and immunity
IS - 8
ER -