TY - JOUR
T1 - Murine autoantibodies to a cryptic membrane antigen
T2 - possible explanation for neuraminidase induced increase in cell immunogenicity
AU - Rosenberg, S. A.
AU - Schwarz, S.
PY - 1974/1/1
Y1 - 1974/1/1
N2 - The normal sera of several mouse strains contained cytotoxic activity toward lymphocytes treated with neuraminidase (Nase) but not to untreated cells. The greatest cytotoxic activity was in C3H/HeJ mice. CBA/J and AKR/J mice also had high levels. In C57BL/6J mice, there was no cytotoxic activity toward Nase treated lymphocytes. This cytotoxic activity (probably antibody) was complement dependent and stable at 56°C for 30 min, but was inactivated by heating to 80°C for 30 min. Nase treated C3H/HeJ and C57BL/6J cells contained equal amounts of this 'hidden' antigen, as determined by absorption studies. Untreated cells contained no detectable antigen. These natural antibodies to a 'cryptic' antigen exposed by Nase treatment were the basis of a theory to explain the increased immunogenicity of Nase treated cells.
AB - The normal sera of several mouse strains contained cytotoxic activity toward lymphocytes treated with neuraminidase (Nase) but not to untreated cells. The greatest cytotoxic activity was in C3H/HeJ mice. CBA/J and AKR/J mice also had high levels. In C57BL/6J mice, there was no cytotoxic activity toward Nase treated lymphocytes. This cytotoxic activity (probably antibody) was complement dependent and stable at 56°C for 30 min, but was inactivated by heating to 80°C for 30 min. Nase treated C3H/HeJ and C57BL/6J cells contained equal amounts of this 'hidden' antigen, as determined by absorption studies. Untreated cells contained no detectable antigen. These natural antibodies to a 'cryptic' antigen exposed by Nase treatment were the basis of a theory to explain the increased immunogenicity of Nase treated cells.
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U2 - 10.1093/jnci/52.4.1151
DO - 10.1093/jnci/52.4.1151
M3 - Article
C2 - 4826586
AN - SCOPUS:0016380613
SN - 0027-8874
VL - 52
SP - 1151
EP - 1155
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 4
ER -