TY - JOUR
T1 - Multistage Adipose-Derived Stem Cell Myogenesis
T2 - An Experimental and Modeling Study
AU - Huri, Pinar Yilgor
AU - Wang, Andrew
AU - Spector, Alexander A.
AU - Grayson, Warren L.
N1 - Funding Information:
We would like to thank Dr. Jeffrey Gimble for providing the ASCs and Dr. Douglas DiGirolamo for use of the Flexcell system. We also thank Sue Kulason for the computational work at early stages of the project. This work was supported by Maryland Stem Cell Research Fund (2012-MSCRFF-165) and Johns Hopkins Department of Biomedical Engineering.
Publisher Copyright:
© 2014, Biomedical Engineering Society.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2014/12
Y1 - 2014/12
N2 - Adipose-derived stem/stromal cells (ASCs) possess great potential as an autologous cell source for cell-based regenerative therapies. We have previously shown that mimicking the natural dynamic muscle loading patterns enhances differentiation capacity of ASCs into aligned myotubes. In particular, the application of uniaxial cyclic strain significantly increased ASC myogenesis in monolayer cultures. In this study, we demonstrate that the temporal expression of key myogenic markers Pax3/7, Desmin, MyoD and myosin heavy chain closely mimics patterns described for muscle satellite cells. Using these lineage markers, we propose that the progression from undifferentiated ASCs to myotubes can be described as transitions through discrete stages. Based on our experimental data, we developed a compartmental kinetic stage-transition model to provide a quantitative description of the differentiation of ASCs to terminally differentiated myotubes. The model describing ASCs’ myogenic differentiation in response to biophysical cues could help to obtain a deeper understanding of factors governing the biological responses and provide clues for experimental methods to increase the efficiency of ASC myogenesis for the development of improved muscle regenerative therapies.
AB - Adipose-derived stem/stromal cells (ASCs) possess great potential as an autologous cell source for cell-based regenerative therapies. We have previously shown that mimicking the natural dynamic muscle loading patterns enhances differentiation capacity of ASCs into aligned myotubes. In particular, the application of uniaxial cyclic strain significantly increased ASC myogenesis in monolayer cultures. In this study, we demonstrate that the temporal expression of key myogenic markers Pax3/7, Desmin, MyoD and myosin heavy chain closely mimics patterns described for muscle satellite cells. Using these lineage markers, we propose that the progression from undifferentiated ASCs to myotubes can be described as transitions through discrete stages. Based on our experimental data, we developed a compartmental kinetic stage-transition model to provide a quantitative description of the differentiation of ASCs to terminally differentiated myotubes. The model describing ASCs’ myogenic differentiation in response to biophysical cues could help to obtain a deeper understanding of factors governing the biological responses and provide clues for experimental methods to increase the efficiency of ASC myogenesis for the development of improved muscle regenerative therapies.
KW - Adipose-derived stem cell
KW - Dynamic culture
KW - Kinetic stage-transition model
KW - Myogenesis
KW - Uniaxial strain
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U2 - 10.1007/s12195-014-0362-7
DO - 10.1007/s12195-014-0362-7
M3 - Article
AN - SCOPUS:84919906807
SN - 1865-5025
VL - 7
SP - 497
EP - 509
JO - Cellular and Molecular Bioengineering
JF - Cellular and Molecular Bioengineering
IS - 4
ER -