Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes

Yi Ping Ho; Matthew C. Kung; Samuel Yang; Tza Huei Wang

doi:10.1021/nl050888v

Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes

Yi Ping Ho, Matthew C. Kung, Samuel Yang, Tza Huei Wang

Whiting School of Engineering

Research output: Contribution to journal › Article › peer-review

152 Scopus citations

Abstract

We demonstrate a hybridization detection method using multicolor oligonucleotide-functionalized quantum dots as nanoprobes. In the presence of various target sequences, combinatorial self-assembly of the nanoprobes via independent hybridization reactions leads to the generation of discernible sequence-specific spectral codings. Detection of single-molecule hybridization is achieved by measuring colocalization of individual nanoprobes. Genetic analysis for anthrax pathogenicity through simultaneous detection of multiple relevant sequences is demonstrated using this novel biosensing method as proof-of-concept.

Original language	English (US)
Pages (from-to)	1693-1697
Number of pages	5
Journal	Nano Letters
Volume	5
Issue number	9
DOIs	https://doi.org/10.1021/nl050888v
State	Published - Sep 1 2005

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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abstract = "We demonstrate a hybridization detection method using multicolor oligonucleotide-functionalized quantum dots as nanoprobes. In the presence of various target sequences, combinatorial self-assembly of the nanoprobes via independent hybridization reactions leads to the generation of discernible sequence-specific spectral codings. Detection of single-molecule hybridization is achieved by measuring colocalization of individual nanoprobes. Genetic analysis for anthrax pathogenicity through simultaneous detection of multiple relevant sequences is demonstrated using this novel biosensing method as proof-of-concept.",

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AB - We demonstrate a hybridization detection method using multicolor oligonucleotide-functionalized quantum dots as nanoprobes. In the presence of various target sequences, combinatorial self-assembly of the nanoprobes via independent hybridization reactions leads to the generation of discernible sequence-specific spectral codings. Detection of single-molecule hybridization is achieved by measuring colocalization of individual nanoprobes. Genetic analysis for anthrax pathogenicity through simultaneous detection of multiple relevant sequences is demonstrated using this novel biosensing method as proof-of-concept.

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Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes

Abstract

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