Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes

Yi Ping Ho, Matthew C. Kung, Samuel Yang, Tza Huei Wang

Research output: Contribution to journalArticlepeer-review

152 Scopus citations


We demonstrate a hybridization detection method using multicolor oligonucleotide-functionalized quantum dots as nanoprobes. In the presence of various target sequences, combinatorial self-assembly of the nanoprobes via independent hybridization reactions leads to the generation of discernible sequence-specific spectral codings. Detection of single-molecule hybridization is achieved by measuring colocalization of individual nanoprobes. Genetic analysis for anthrax pathogenicity through simultaneous detection of multiple relevant sequences is demonstrated using this novel biosensing method as proof-of-concept.

Original languageEnglish (US)
Pages (from-to)1693-1697
Number of pages5
JournalNano Letters
Issue number9
StatePublished - Sep 1 2005

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering


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