TY - GEN
T1 - Multiplex droplet platform for rapid single-cell antibiogram
AU - Zhang, Pengfei
AU - Kaushik, Aniruddha
AU - Hsieh, Kuangwen
AU - Wang, Tza Huei
N1 - Funding Information:
This work was financially supported by the National Institutes of Health (R01AI117032, R01AI137272, R01AI138978) and National Science Foundation (1538813). REFERENCES [1] D. J. Shin, N. Andini, K. Hsieh, S. Yang and T.-H. Wang, Annu. Rev. Anal. Chem., 2019, 12, annurev-anchem-061318-115529. [2] M. Davenport, K. E. Mach, L. M. D. Shortliffe, N. Banaei, T. H. Wang and J. C. Liao, Nat. Rev. Urol., 2017, 14, 298–310. [3] A. M. Kaushik, K. Hsieh, L. Chen, D. J. Shin, J. C. Liao and T. H. Wang, Biosens. Bioelectron., 2017, 97, 260–266. [4] P. Zhang, A. Kaushik, K. Hsieh and T.-H. Wang, Microsystems Nanoeng., 2019, 5, 22. CONTACT * Tza-Huei Wang; phone: +1-410-516-7086; [email protected]
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - This paper describes a droplet microfluidic platform capable of rapidly generating a comprehensive antibiogram for pathogenic bacteria against commonly administered antibiotics. Our platform assembles picoliter droplet groups that each digitizes single bacterial cells along with a distinct antibiotic at a predefined dosage. After 85 min. of on-chip incubation, we can assess the effect of multiple antibiotics on the bacteria via a fluorescent single-cell viability test. Our platform achieves rapid antibiotic susceptibility test (AST) for four antibiotics in 2.5 hours, which can potentially guide clinical practice in the future.
AB - This paper describes a droplet microfluidic platform capable of rapidly generating a comprehensive antibiogram for pathogenic bacteria against commonly administered antibiotics. Our platform assembles picoliter droplet groups that each digitizes single bacterial cells along with a distinct antibiotic at a predefined dosage. After 85 min. of on-chip incubation, we can assess the effect of multiple antibiotics on the bacteria via a fluorescent single-cell viability test. Our platform achieves rapid antibiotic susceptibility test (AST) for four antibiotics in 2.5 hours, which can potentially guide clinical practice in the future.
KW - Antibiogram
KW - Antibiotic Susceptibility Test (AST)
KW - Droplet
KW - Multiplex
KW - Single-cell
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M3 - Conference contribution
AN - SCOPUS:85094966469
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 120
EP - 121
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -