@article{37be0cebee1e41449d96e1684cc1a7ed,
title = "Multiple Sclerosis Is Associated with Immunoglobulin Germline Gene Variation of Transitional B Cells",
abstract = "The regulatory functions of the B-cell compartment play an important role in the development and suppression of the immune response. Disruption of their anti-inflammatory functions may lead to the acceleration of immunopathological processes, and to autoimmune diseases, in particular. Unfortunately, the exact mechanism underlying the functioning and development of regulatory B cells (Breg) has not yet been fully elucidated. Almost nothing is known about their specificity and the structure of their B-cell receptors (BCRs). In this research, we analyzed the BCR repertoire of the transitional Breg (tBreg) subpopulation with the CD19+CD24highCD38high phenotype in patients with multiple sclerosis (MS), using next-generation sequencing (NGS). We show, for the first time, that the immunoglobulin germline distribution in the tBreg subpopulation is different between MS patients and healthy donors. The registered variation was more significant in patients with a more severe form of the disease, highly active MS (HAMS), compared to those with benign MS (BMS).",
keywords = "BCR-Seq, NGS, TrB, germline, immunoglobulin, multiple sclerosis, neurodegeneration, regulatory B cells, transitional B cells",
author = "Lomakin, {Y. A.} and Ovchinnikova, {L. A.} and Zakharova, {M. N.} and Ivanova, {M. V.} and Simaniv, {T. O.} and Kabilov, {M. R.} and Bykova, {N. A.} and Mukhina, {V. S.} and Kaminskaya, {A. N.} and Tupikin, {A. E.} and Zakharova, {M. Y.} and Favorov, {A. V.} and Illarioshkin, {S. N.} and Belogurov, {A. A.} and Gabibov, {A. G.}",
note = "Funding Information: Cell sorting was carried out using the equipment provided by the Center for Collective Use of the Institute of Bioorganic Chemistry and supported by the Ministry of Science and Higher Education of the Russian Federation (project No. 075-15-2020-807). Funding Information: This study was supported by the Russian Science Foundation (grant No.18-74-10079 “Self-assembled genetically encoded nanocages as a tool for the treatment of multiple sclerosis”), the Russian Foundation for Basic Research grant No. 17-00-00229 (obtaining peripheral blood of patients with multiple sclerosis and healthy donors) and project No. 075-15-2021-1033 (13.2251.21.0111) in terms of large-scale sequencing. Publisher Copyright: {\textcopyright} 2022 National Research University Higher School of Economics. This is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited",
year = "2022",
doi = "10.32607/actanaturae.11794",
language = "English (US)",
volume = "14",
pages = "84--93",
journal = "Acta Naturae",
issn = "2075-8251",
publisher = "Acta Naturae",
number = "4-55",
}