TY - JOUR
T1 - Multiple mitochondrial DNA polymerases in Trypanosoma brucei
AU - Klingbeil, Michele M.
AU - Motyka, Shawn A.
AU - Englund, Paul T.
N1 - Funding Information:
We dedicate this paper to the memory of Viiu Klein. We appreciate comments on the manuscript by Drs. D. Robinson, H. Sesaki, T. Shapiro, B. Sollner-Webb, and members of our lab. M.M.K. was supported by NRSA (5F32AI09789). This work was supported by the National Institutes of Health (GM27608).
PY - 2002
Y1 - 2002
N2 - Kinetoplast DNA (kDNA), the unusual mitochondrial DNA of Trypanosoma brucei, is a network containing thousands of catenated circles. Database searching for a kDNA replicative polymerase (pol) revealed no mitochondrial pol γ homolog. Instead, we identified four proteins (TbPOLIA, IB, IC, and ID) related to bacterial pol I. Remarkably, all four localized to the mitochondrion. TbPOLIB and TbPOLIC localized beside the kDNA where replication occurs, and their knockdown by RNA interference caused kDNA network shrinkage. Furthermore, silencing of TbPOLIC caused loss of both minicircles and maxicircles and accumulation of minicircle replication intermediates, consistent with a role in replication. While typical mitochondria contain one DNA polymerase, pol γ, trypanosome mitochondria contain five such enzymes, including the previously characterized pol β.
AB - Kinetoplast DNA (kDNA), the unusual mitochondrial DNA of Trypanosoma brucei, is a network containing thousands of catenated circles. Database searching for a kDNA replicative polymerase (pol) revealed no mitochondrial pol γ homolog. Instead, we identified four proteins (TbPOLIA, IB, IC, and ID) related to bacterial pol I. Remarkably, all four localized to the mitochondrion. TbPOLIB and TbPOLIC localized beside the kDNA where replication occurs, and their knockdown by RNA interference caused kDNA network shrinkage. Furthermore, silencing of TbPOLIC caused loss of both minicircles and maxicircles and accumulation of minicircle replication intermediates, consistent with a role in replication. While typical mitochondria contain one DNA polymerase, pol γ, trypanosome mitochondria contain five such enzymes, including the previously characterized pol β.
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U2 - 10.1016/S1097-2765(02)00571-3
DO - 10.1016/S1097-2765(02)00571-3
M3 - Article
C2 - 12150917
AN - SCOPUS:0036342502
SN - 1097-2765
VL - 10
SP - 175
EP - 186
JO - Molecular cell
JF - Molecular cell
IS - 1
ER -