TY - JOUR
T1 - Multiple activities of a cloned cell line mediating natural killer cell function
AU - Nabel, G.
AU - Bucalo, L. R.
AU - Allard, J.
AU - Wigzell, H.
AU - Cantor, H.
PY - 1981
Y1 - 1981
N2 - A special class of immunologic cells can lyse or damage a variety of target cells, notably malignant cells in vitro. These cells have been called natural killer (NK) cells because lysis does not require deliberate immunization by tumor cells. Although these cells can be distinguished from conventional T cells, B cells, and phagocytic cells, they have been difficult to define. The authors describe a representative cloned cell line that was obtained by cloning Ig -Ly-5 + cells from spleen. This clone, Cl.Ly-1 -2 -NK-1 +/11, displays Thy-1, Ly-5, Qat-4, Qat-5, and NK-1 cell surface antigens and lyses the NK-sensitive TAC-1 lymphoma cells, but does not lyse-RL-12 cells, and NK-resistant lymphoma. In addition, this clone lysed the P815 mastocytoma, EL4 lymphoma, and lipopolysaccharide-activated B lymphocyte targets. This cloned population therefore combined information for a unique display of cell surface antigens and specialized function similar to 'activated' NK cells. Because this cloned population forms conjugates with susceptible but not resistant target cells, it may prove useful to identify the structure of cell surface molecules that recognize foreign cells. Finally, cells of this clone also specifically lysed target cells coated by antibodies to determinants on the target cell surface, demonstrating that a single-cloned cell population can mediate two specialized immunologic functions: antibody-dependent cellular cytotoxicity and NK cell lysis.
AB - A special class of immunologic cells can lyse or damage a variety of target cells, notably malignant cells in vitro. These cells have been called natural killer (NK) cells because lysis does not require deliberate immunization by tumor cells. Although these cells can be distinguished from conventional T cells, B cells, and phagocytic cells, they have been difficult to define. The authors describe a representative cloned cell line that was obtained by cloning Ig -Ly-5 + cells from spleen. This clone, Cl.Ly-1 -2 -NK-1 +/11, displays Thy-1, Ly-5, Qat-4, Qat-5, and NK-1 cell surface antigens and lyses the NK-sensitive TAC-1 lymphoma cells, but does not lyse-RL-12 cells, and NK-resistant lymphoma. In addition, this clone lysed the P815 mastocytoma, EL4 lymphoma, and lipopolysaccharide-activated B lymphocyte targets. This cloned population therefore combined information for a unique display of cell surface antigens and specialized function similar to 'activated' NK cells. Because this cloned population forms conjugates with susceptible but not resistant target cells, it may prove useful to identify the structure of cell surface molecules that recognize foreign cells. Finally, cells of this clone also specifically lysed target cells coated by antibodies to determinants on the target cell surface, demonstrating that a single-cloned cell population can mediate two specialized immunologic functions: antibody-dependent cellular cytotoxicity and NK cell lysis.
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U2 - 10.1084/jem.153.6.1582
DO - 10.1084/jem.153.6.1582
M3 - Article
C2 - 6973001
AN - SCOPUS:0019509542
SN - 0022-1007
VL - 153
SP - 1582
EP - 1591
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 6
ER -