Multiple actions of N-methyl-d-aspartate on cat neocortical neurons in vitro

J. A. Flatman; P. C. Schwindt; W. E. Crill; C. E. Stafstrom

doi:10.1016/0006-8993(83)91323-9

Multiple actions of N-methyl-d-aspartate on cat neocortical neurons in vitro

J. A. Flatman, P. C. Schwindt, W. E. Crill, C. E. Stafstrom

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

The potent excitatory amino acid receptor agonist, N-methyl-d-aspartate (NMDA), was applied to cat neocortical neurons in an in vitro slice preparation. NMDA evokes a slow depolarization with a net input conductance decrease, repetitive firing, rhythmic depolarization shifts and bi-stable membrane potential behavior. Use of blocking agents, ion substitution and voltage clamp indicates that NMDA induces a highly voltage-dependent TTX-resistant inward sodium current which accounts for much of the NMDA response.

Original language	English (US)
Pages (from-to)	169-173
Number of pages	5
Journal	Brain research
Volume	266
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)91323-9
State	Published - Apr 25 1983
Externally published	Yes

Keywords

N-methyl-d-aspartate
depolarization shift
inward rectification
neocortical neurons
transmitter-sensitive conductance

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(83)91323-9

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title = "Multiple actions of N-methyl-d-aspartate on cat neocortical neurons in vitro",

abstract = "The potent excitatory amino acid receptor agonist, N-methyl-d-aspartate (NMDA), was applied to cat neocortical neurons in an in vitro slice preparation. NMDA evokes a slow depolarization with a net input conductance decrease, repetitive firing, rhythmic depolarization shifts and bi-stable membrane potential behavior. Use of blocking agents, ion substitution and voltage clamp indicates that NMDA induces a highly voltage-dependent TTX-resistant inward sodium current which accounts for much of the NMDA response.",

keywords = "N-methyl-d-aspartate, depolarization shift, inward rectification, neocortical neurons, transmitter-sensitive conductance",

author = "Flatman, {J. A.} and Schwindt, {P. C.} and Crill, {W. E.} and Stafstrom, {C. E.}",

note = "Funding Information: Supported by the Danish Medical Research Council (J.A.F.), the Veterans Administration and NIH Grants NS 16792 (W.E.C. and P.C.S.), GM 07266 (C.E.S.), and Epilepsy Foundation of America. We thank Gregg Hinz and David Johnson for technical assistance.",

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T1 - Multiple actions of N-methyl-d-aspartate on cat neocortical neurons in vitro

AU - Flatman, J. A.

AU - Schwindt, P. C.

AU - Crill, W. E.

AU - Stafstrom, C. E.

N1 - Funding Information: Supported by the Danish Medical Research Council (J.A.F.), the Veterans Administration and NIH Grants NS 16792 (W.E.C. and P.C.S.), GM 07266 (C.E.S.), and Epilepsy Foundation of America. We thank Gregg Hinz and David Johnson for technical assistance.

PY - 1983/4/25

Y1 - 1983/4/25

N2 - The potent excitatory amino acid receptor agonist, N-methyl-d-aspartate (NMDA), was applied to cat neocortical neurons in an in vitro slice preparation. NMDA evokes a slow depolarization with a net input conductance decrease, repetitive firing, rhythmic depolarization shifts and bi-stable membrane potential behavior. Use of blocking agents, ion substitution and voltage clamp indicates that NMDA induces a highly voltage-dependent TTX-resistant inward sodium current which accounts for much of the NMDA response.

AB - The potent excitatory amino acid receptor agonist, N-methyl-d-aspartate (NMDA), was applied to cat neocortical neurons in an in vitro slice preparation. NMDA evokes a slow depolarization with a net input conductance decrease, repetitive firing, rhythmic depolarization shifts and bi-stable membrane potential behavior. Use of blocking agents, ion substitution and voltage clamp indicates that NMDA induces a highly voltage-dependent TTX-resistant inward sodium current which accounts for much of the NMDA response.

KW - N-methyl-d-aspartate

KW - depolarization shift

KW - inward rectification

KW - neocortical neurons

KW - transmitter-sensitive conductance

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Multiple actions of N-methyl-d-aspartate on cat neocortical neurons in vitro

Abstract

Keywords

ASJC Scopus subject areas

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