Multiinstitution study of non-Hodgkin's lymphomas using frozen section immunoperoxidase: The Southeastern Cancer Study Group experience

M. J. Borowitz, S. Newby, R. K. Brynes, J. B. Cousar, C. C. Whitcomb, J. D. Crissman, G. E. Byrne, R. D. Collins

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


This report describes the experience of the Southeastern Cancer Study Group (SECSG) with a transport medium used for immunologic phenotyping of non-Hodgkin's lymphomas. In a 2-mo pilot study, portions of 53 specimens of non-Hodgkin's lymphoma from 4 member institutions of the SECSG and affiliated community hospitals were sent by regular mail to a central laboratory. Immunologic phenotyping was carried out using a frozen section immunoperoxidase technique. In 48 of the cases, a clear-cut immunologic phenotype was obtained. Thirty-four tumors were of B cell origin and 7 had T cell markers. Six of the remaining lymphomas had neither B nor T cell markers, and the 7th had both. In 12 cases, phenotyping was also carried out at the originating institution using conventional cell suspension techniques; agreement between the 2 methods was excellent. The immunologic results were correlated with histopathologic diagnosis standardized using the Working Formulation for non-Hodgkin's lymphomas. It was found that the low grade tumors were all B cell, but that the intermediate grade tumors were very heterogeneous immunologically. About one-fourth of the diffuse, intermediate grade or miscellaneous tumors had T cell markers. Our results indicate that immunologic phenotyping may be performed satisfactorily on transported material, making multiinstitution studies on the prognostic significance of immunologic phenotype in non-Hodgkin's lymphomas feasible.

Original languageEnglish (US)
Pages (from-to)1147-1152
Number of pages6
Issue number5
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology


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