Multifunctional chondroitin sulphate for cartilage tissue-biomaterial integration

Dong An Wang; Shyni Varghese; Blanka Sharma; Iossif Strehin; Sara Fermanian; Justin Gorham; D. Howard Fairbrother; Brett Cascio; Jennifer H. Elisseeff

doi:10.1038/nmat1890

Multifunctional chondroitin sulphate for cartilage tissue-biomaterial integration

Dong An Wang, Shyni Varghese, Blanka Sharma, Iossif Strehin, Sara Fermanian, Justin Gorham, D. Howard Fairbrother, Brett Cascio, Jennifer H. Elisseeff

School of Medicine

Research output: Contribution to journal › Article › peer-review

463 Scopus citations

Abstract

A biologically active, high-strength tissue adhesive is needed for numerous medical applications in tissue engineering and regenerative medicine. Integration of biomaterials or implants with surrounding native tissue is crucial for both immediate functionality and long-term performance of the tissue. Here, we use the biopolymer chondroitin sulphate (CS), one of the major components of cartilage extracellular matrix, to develop a novel bioadhesive that is readily applied and acts quickly. CS was chemically functionalized with methacrylate and aldehyde groups on the polysaccharide backbone to chemically bridge biomaterials and tissue proteins via a twofold covalent link. Three-dimensional hydrogels (with and without cells) bonded to articular cartilage defects. In in vitro and in vivo functional studies this approach led to mechanical stability of the hydrogel and tissue repair in cartilage defects.

Original language	English (US)
Pages (from-to)	385-392
Number of pages	8
Journal	Nature Materials
Volume	6
Issue number	5
DOIs	https://doi.org/10.1038/nmat1890
State	Published - May 2007

ASJC Scopus subject areas

General Chemistry
General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1038/nmat1890

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title = "Multifunctional chondroitin sulphate for cartilage tissue-biomaterial integration",

abstract = "A biologically active, high-strength tissue adhesive is needed for numerous medical applications in tissue engineering and regenerative medicine. Integration of biomaterials or implants with surrounding native tissue is crucial for both immediate functionality and long-term performance of the tissue. Here, we use the biopolymer chondroitin sulphate (CS), one of the major components of cartilage extracellular matrix, to develop a novel bioadhesive that is readily applied and acts quickly. CS was chemically functionalized with methacrylate and aldehyde groups on the polysaccharide backbone to chemically bridge biomaterials and tissue proteins via a twofold covalent link. Three-dimensional hydrogels (with and without cells) bonded to articular cartilage defects. In in vitro and in vivo functional studies this approach led to mechanical stability of the hydrogel and tissue repair in cartilage defects.",

author = "Wang, {Dong An} and Shyni Varghese and Blanka Sharma and Iossif Strehin and Sara Fermanian and Justin Gorham and Fairbrother, {D. Howard} and Brett Cascio and Elisseeff, {Jennifer H.}",

note = "Funding Information: We acknowledge S. Ramaswamy and R. Spencer for MRI access; J. Cooper and S.-h. Moon for use of mechanical analysis facilities; and N. Marcus for assistance in the animal studies and critical review of the manuscript. The authors would like to acknowledge the Materials Science Department at Johns Hopkins University for use of the surface analysis laboratory. This research was financially supported by NIH Grant No. R21 EB002369-01, R01 EB05517-01, the State of Maryland University Technology Development Fund and Cartilix. Correspondence and requests for materials should be addressed to J.H.E. Supplementary Information accompanies this paper on www.nature.com/naturematerials.",

year = "2007",

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doi = "10.1038/nmat1890",

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AU - Wang, Dong An

AU - Varghese, Shyni

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AU - Strehin, Iossif

AU - Fermanian, Sara

AU - Gorham, Justin

AU - Fairbrother, D. Howard

AU - Cascio, Brett

AU - Elisseeff, Jennifer H.

N1 - Funding Information: We acknowledge S. Ramaswamy and R. Spencer for MRI access; J. Cooper and S.-h. Moon for use of mechanical analysis facilities; and N. Marcus for assistance in the animal studies and critical review of the manuscript. The authors would like to acknowledge the Materials Science Department at Johns Hopkins University for use of the surface analysis laboratory. This research was financially supported by NIH Grant No. R21 EB002369-01, R01 EB05517-01, the State of Maryland University Technology Development Fund and Cartilix. Correspondence and requests for materials should be addressed to J.H.E. Supplementary Information accompanies this paper on www.nature.com/naturematerials.

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Multifunctional chondroitin sulphate for cartilage tissue-biomaterial integration

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