Multicolor single-molecule FRET for DNA and RNA processes

Xinyu A. Feng, Matthew F. Poyton, Taekjip Ha

Research output: Contribution to journalReview articlepeer-review


Single-molecule fluorescence resonance energy transfer (smFRET) is a useful tool for observing the dynamics of protein–nucleic acid interactions. Although most smFRET measurements have used two fluorophores, multicolor smFRET measurements using more than two fluorophores offer more information about how protein–nucleic acid complexes dynamically move, assemble, and disassemble. Multicolor smFRET experiments include three or more fluorophores and at least one donor-acceptor pair. This review highlights how multicolor smFRET is being used to probe the dynamics of three different classes of biochemical processes—protein-DNA interactions, chromatin remodeling, and protein translation.

Original languageEnglish (US)
Pages (from-to)26-33
Number of pages8
JournalCurrent Opinion in Structural Biology
StatePublished - Oct 2021

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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