Multicenter evaluation of biofire filmarray meningitis/encephalitis panel for detection of bacteria, viruses, and yeast in cerebrospinal fluid specimens

Amy L. Leber, Kathy Everhart, Joan Miquel Balada-Llasat, Jillian Cullison, Judy Daly, Sarah Holt, Paul Lephart, Hossein Salimnia, Paul C. Schreckenberger, Sharon DesJarlais, Sharon L. Reed, Kimberle C. Chapin, Lindsay LeBlanc, J. Kristie Johnson, Nicole L. Soliven, Karen C. Carroll, Jo Anne Miller, Jennifer Dien Bard, Javier Mestas, Matthew BankowskiTori Enomoto, Andrew C. Hemmert, Kevin M. Bourzac

Research output: Contribution to journalArticlepeer-review

234 Scopus citations


Rapid diagnosis and treatment of infectious meningitis and encephalitis are critical to minimize morbidity and mortality. Comprehensive testing of cerebrospinal fluid (CSF) often includes Gram stain, culture, antigen detection, and molecular methods, paired with chemical and cellular analyses. These methods may lack sensitivity or specificity, can take several days, and require significant volume for complete analysis. The FilmArray Meningitis/Encephalitis (ME) Panel is a multiplexed in vitro diagnostic test for the simultaneous, rapid (1-h) detection of 14 pathogens directly from CSF specimens: Escherichia coli K1, Haemophilus influenzae, Listeria monocytogenes, Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus agalactiae, cytomegalovirus, enterovirus, herpes simplex virus 1 and 2, human herpesvirus 6, human parechovirus, varicella-zoster virus, and Cryptococcus neoformans/Cryptococcus gattii. We describe a multicenter evaluation of 1,560 prospectively collected CSF specimens with performance compared to culture (bacterial analytes) and PCR (all other analytes). The FilmArray ME Panel demonstrated a sensitivity or positive percentage of agreement of 100% for 9 of 14 analytes. Enterovirus and human herpesvirus type 6 had agreements of 95.7% and 85.7%, and L. monocytogenes and N. meningitidis were not observed in the study. For S. agalactiae, there was a single false-positive and false-negative result each, for a sensitivity and specificity of 0 and 99.9%, respectively. The specificity or negative percentage of agreement was 99.2% or greater for all other analytes. The FilmArray ME Panel is a sensitive and specific test to aid in diagnosis of ME. With use of this comprehensive and rapid test, improved patient outcomes and antimicrobial stewardship are anticipated.

Original languageEnglish (US)
Pages (from-to)2251-2261
Number of pages11
JournalJournal of clinical microbiology
Issue number9
StatePublished - Sep 2016

ASJC Scopus subject areas

  • Microbiology (medical)


Dive into the research topics of 'Multicenter evaluation of biofire filmarray meningitis/encephalitis panel for detection of bacteria, viruses, and yeast in cerebrospinal fluid specimens'. Together they form a unique fingerprint.

Cite this