MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy

Olivier Hauger, Emma E. Frost, Ruud Van Heeswijk, Colette Deminière, R. Xue, Yahsou Delmas, Christian Combe, Chrit T.W. Moonen, Nicolas Grenier, Jeff W.M. Bulte

Research output: Contribution to journalArticlepeer-review

112 Scopus citations


Purpose: To assess renal glomerular homing of intravenously injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs) at in vivo and ex vivo magnetic resonance (MR) imaging in an experimental rat model of mesangiolysis. Materials and Methods: Animal procedures were performed in accordance with protocols approved by Institutional Animal Care and Use Committee. Fourteen rats were divided into two groups: one pathologic (n = 10), with persistent mesangiolysis following simultaneous injection of OX-7 monoclonal antibody and puromycin aminonucleoside in which 107 SPIO-and DiI-labeled MSCs were injected, and one control (n = 4). In vivo and ex-vivo MR imaging examinations were performed with 4.7- and 9.4-T spectrometers, respectively, and T2*-weighted sequences. In vivo signal intensity variations were measured in the liver and kidney before and 6 days after MSC injection. Intrarenal signal intensity variations were correlated with histopathologic data by means of colocalization of DiI fluorescence, α-actin, and Prussian blue stain-positive cells. Histologic differences between the glomerular homing of MSCs in different kidney portions were correlated to the areas of MR signal intensity decrease with nonparametric statistical tests. Results: On in vivo images, signal intensity measurements of pathologic kidneys following MSC injection did not show any signal intensity decrease (P = .7), whereas a 34% ± 14 (mean ± standard deviation) signal intensity decrease was observed in the liver (P < .01), where a substantial number of labeled cells were trapped. On ex vivo images, pathologic kidneys showed focal cortical (glomerular) areas of signal intensity loss, which was absent in controls. The areas of low signal intensity correlated well with α-actin and Prussian blue stain- and DiI-positive areas (P < .01), which indicates that MSCs specifically home to injured tissue. No MSCs were detected in the kidneys of control animals. Conclusion: Intravenously injected MSCs specifically home to focal areas of glomerular damage and can be detected at ex vivo MR imaging.

Original languageEnglish (US)
Pages (from-to)200-210
Number of pages11
Issue number1
StatePublished - Jan 2006

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


Dive into the research topics of 'MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy'. Together they form a unique fingerprint.

Cite this