MPtopo: A database of membrane protein topology

Sajith Jayasinghe, Kalina Hristova, Stephen H. White

Research output: Contribution to journalArticlepeer-review

141 Scopus citations


The reliability of the transmembrane (TM) sequence assignments for membrane proteins (MPs) in standard sequence databases is uncertain because the vast majority are based on hydropathy plots. A database of MPs with dependable assignments is necessary for developing new computational tools for the prediction of MP structure. We have therefore created MPtopo, a database of MPs whose topologies have been verified experimentally by means of crystallography, gene fusion, and other methods. Tests using MPtopo strongly validated four existing MP topology-prediction algorithms. MPtopo is freely available over the internet and can be queried by means of an SQL-based search engine.

Original languageEnglish (US)
Pages (from-to)455-458
Number of pages4
JournalProtein Science
Issue number2
StatePublished - 2001
Externally publishedYes


  • Hydropathy plots
  • Prediction accuracy
  • Protein structure prediction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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