TY - JOUR
T1 - Mouse model of post-surgical breakdown of the blood-retinal barrier
AU - Liu, Hansheng
AU - Demetriades, Anna Maria
AU - Xiao, Wei Hong
AU - Campochiaro, Peter A.
AU - Vinores, Stanley A.
N1 - Funding Information:
This study was supported by grants from the National Eye Institute [EY10017 (SAV) and core grant P30EY1765]; Research to Prevent Blindness [Lew R. Wasserman Merit Awards (PAC, SAV) and an unrestricted grant], the American Diabetes Association and European Association for the Study of Diabetes (Trans-Atlantic Fellowship) (AMD); Knights Templar Eye Foundation, Inc. (AMD); the BMA John William Clark Award (AMD); and Dr. and Mrs. William Lake. PAC is the George S. and Dolores Dore Eccles Professor of Ophthalmology and Neuroscience.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/6
Y1 - 2004/6
N2 - Purpose. Post-surgical macular edema is an important clinical problem resulting from breakdown of the blood-retinal barrier (BRB) after surgery. This study was designed to develop a mouse model of post-surgical BRB breakdown. Methods. Two 25-gauge needles, one for infusion and one for aspiration, were inserted through the limbus and into the lens of one eye of adult male C57BL/6 mice. The anterior portion of the lens was aspirated and the fellow eye was untreated. At several time points after surgery, the integrity of the BRB was assessed quantitatively, using [3H]mannitol as a tracer, or qualitatively, using immunohistochemical staining for albumin. Results. Eyes with partial lens extraction had a significant increase in retinal vascular leakage one day after surgery, which persisted two and three days after surgery, but by five days, was not significantly different from controls. Immunohistochemical staining for albumin demonstrated that the breech in the barrier was sufficient to allow passage of a 60 kDa protein into the retina, and was localized predominantly to retinal vessels. Conclusions. Partial lens extraction in mice results in BRB breakdown (primarily the inner BRB) that is highly reproducible in the severity of leakage and its time course. This provides a valuable tool for investigation of the molecular pathogenesis and new treatment approaches for post-surgical breakdown of the BRB.
AB - Purpose. Post-surgical macular edema is an important clinical problem resulting from breakdown of the blood-retinal barrier (BRB) after surgery. This study was designed to develop a mouse model of post-surgical BRB breakdown. Methods. Two 25-gauge needles, one for infusion and one for aspiration, were inserted through the limbus and into the lens of one eye of adult male C57BL/6 mice. The anterior portion of the lens was aspirated and the fellow eye was untreated. At several time points after surgery, the integrity of the BRB was assessed quantitatively, using [3H]mannitol as a tracer, or qualitatively, using immunohistochemical staining for albumin. Results. Eyes with partial lens extraction had a significant increase in retinal vascular leakage one day after surgery, which persisted two and three days after surgery, but by five days, was not significantly different from controls. Immunohistochemical staining for albumin demonstrated that the breech in the barrier was sufficient to allow passage of a 60 kDa protein into the retina, and was localized predominantly to retinal vessels. Conclusions. Partial lens extraction in mice results in BRB breakdown (primarily the inner BRB) that is highly reproducible in the severity of leakage and its time course. This provides a valuable tool for investigation of the molecular pathogenesis and new treatment approaches for post-surgical breakdown of the BRB.
KW - Blood-retinal barrier
KW - Macular edema
KW - Mouse model
KW - Ocular surgery
KW - Vasopermeability factors
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U2 - 10.1080/02713680490503769
DO - 10.1080/02713680490503769
M3 - Article
C2 - 15512950
AN - SCOPUS:4143058321
SN - 0271-3683
VL - 28
SP - 421
EP - 426
JO - Current Eye Research
JF - Current Eye Research
IS - 6
ER -