Mouse mast cells express the tryptic protease neuropsin/Prss19

Guang W. Wong; Yi Yang; Shinsuke Yasuda; Lixin Li; Richard L. Stevens

doi:10.1016/S0006-291X(03)00339-5

Mouse mast cells express the tryptic protease neuropsin/Prss19

Guang W. Wong, Yi Yang, Shinsuke Yasuda, Lixin Li, Richard L. Stevens

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The only tryptic enzymes identified so far in mouse mast cells (MCs) are three members of the chromosome 17A3.3 family of neutral proteases. Sequence analysis of a cDNA library revealed that BALB/c mouse bone marrow-derived MCs express neuropsin, a member of the chromosome 7B2 family of tryptic kallikreins. Kinetic studies revealed that neuropsin is expressed relatively early in MC development. As assessed immunohistochemically, the MCs residing in numerous connective tissues store neuropsin in their secretory granules. The finding that the neuropsin transcript is maximally expressed in the intestine at the height of a helminth infection indicates that MC-committed progenitors selectively increase their expression of neuropsin as they develop into mature mucosal MCs. This is the first report documenting the expression of neuropsin in an immune cell. Thus, it is now apparent that mouse MCs store at least two distinct families of tryptic-like proteases in their secretory granules.

Original language	English (US)
Pages (from-to)	320-325
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	303
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(03)00339-5
State	Published - Mar 28 2003
Externally published	Yes

Keywords

Kallikreins
Mast cells
Neuropsin
Prss19
Serine proteases
Tryptases

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(03)00339-5

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title = "Mouse mast cells express the tryptic protease neuropsin/Prss19",

abstract = "The only tryptic enzymes identified so far in mouse mast cells (MCs) are three members of the chromosome 17A3.3 family of neutral proteases. Sequence analysis of a cDNA library revealed that BALB/c mouse bone marrow-derived MCs express neuropsin, a member of the chromosome 7B2 family of tryptic kallikreins. Kinetic studies revealed that neuropsin is expressed relatively early in MC development. As assessed immunohistochemically, the MCs residing in numerous connective tissues store neuropsin in their secretory granules. The finding that the neuropsin transcript is maximally expressed in the intestine at the height of a helminth infection indicates that MC-committed progenitors selectively increase their expression of neuropsin as they develop into mature mucosal MCs. This is the first report documenting the expression of neuropsin in an immune cell. Thus, it is now apparent that mouse MCs store at least two distinct families of tryptic-like proteases in their secretory granules.",

keywords = "Kallikreins, Mast cells, Neuropsin, Prss19, Serine proteases, Tryptases",

author = "Wong, {Guang W.} and Yi Yang and Shinsuke Yasuda and Lixin Li and Stevens, {Richard L.}",

note = "Funding Information: This work was supported by NIH Grants HL-36110 and HL-63284. We thank Drs. Sadao Shiosaka and Kazumasa Matsumoto (Division of Structural Cell Biology, Nara Institute of Science and Technology, Nara, Japan) for providing the rat monoclonal antibody that recognizes mouse neuropsin.",

year = "2003",

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doi = "10.1016/S0006-291X(03)00339-5",

language = "English (US)",

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TY - JOUR

T1 - Mouse mast cells express the tryptic protease neuropsin/Prss19

AU - Wong, Guang W.

AU - Yang, Yi

AU - Yasuda, Shinsuke

AU - Li, Lixin

AU - Stevens, Richard L.

N1 - Funding Information: This work was supported by NIH Grants HL-36110 and HL-63284. We thank Drs. Sadao Shiosaka and Kazumasa Matsumoto (Division of Structural Cell Biology, Nara Institute of Science and Technology, Nara, Japan) for providing the rat monoclonal antibody that recognizes mouse neuropsin.

PY - 2003/3/28

Y1 - 2003/3/28

N2 - The only tryptic enzymes identified so far in mouse mast cells (MCs) are three members of the chromosome 17A3.3 family of neutral proteases. Sequence analysis of a cDNA library revealed that BALB/c mouse bone marrow-derived MCs express neuropsin, a member of the chromosome 7B2 family of tryptic kallikreins. Kinetic studies revealed that neuropsin is expressed relatively early in MC development. As assessed immunohistochemically, the MCs residing in numerous connective tissues store neuropsin in their secretory granules. The finding that the neuropsin transcript is maximally expressed in the intestine at the height of a helminth infection indicates that MC-committed progenitors selectively increase their expression of neuropsin as they develop into mature mucosal MCs. This is the first report documenting the expression of neuropsin in an immune cell. Thus, it is now apparent that mouse MCs store at least two distinct families of tryptic-like proteases in their secretory granules.

AB - The only tryptic enzymes identified so far in mouse mast cells (MCs) are three members of the chromosome 17A3.3 family of neutral proteases. Sequence analysis of a cDNA library revealed that BALB/c mouse bone marrow-derived MCs express neuropsin, a member of the chromosome 7B2 family of tryptic kallikreins. Kinetic studies revealed that neuropsin is expressed relatively early in MC development. As assessed immunohistochemically, the MCs residing in numerous connective tissues store neuropsin in their secretory granules. The finding that the neuropsin transcript is maximally expressed in the intestine at the height of a helminth infection indicates that MC-committed progenitors selectively increase their expression of neuropsin as they develop into mature mucosal MCs. This is the first report documenting the expression of neuropsin in an immune cell. Thus, it is now apparent that mouse MCs store at least two distinct families of tryptic-like proteases in their secretory granules.

KW - Kallikreins

KW - Mast cells

KW - Neuropsin

KW - Prss19

KW - Serine proteases

KW - Tryptases

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JO - Biochemical and Biophysical Research Communications

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Mouse mast cells express the tryptic protease neuropsin/Prss19

Abstract

Keywords

ASJC Scopus subject areas

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