TY - JOUR
T1 - Mouse allergen exposure and immunologic responses
T2 - IgE-mediated mouse sensitization and mouse specific IgG and IgG4 levels
AU - Matsui, Elizabeth C.
AU - Krop, Esmeralda J.M.
AU - Diette, Gregory B.
AU - Aalberse, Rob C.
AU - Smith, Abigail L.
AU - Eggleston, Peyton A.
N1 - Funding Information:
* Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland. † Department of Pulmonology, Academic Medical Center, University of Amsterdam, Amersterdam, the Netherlands. ‡ Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland. § Department of Allergy, CLB Sanquin Blood Supply Foundation, Amsterdam, the Netherlands. ¶ The Jackson Laboratory, Bar Harbor, Maine. Supported by AI07007 from the National Institute of Allergy and Infectious Diseases; RR 12552 from the National Center for Research Resources, National Institutes of Health; ES09606 from the National Institute of Environmental Health Sciences; R82674 from the Environmental Protection Agency; HL058942 from the National Heart, Lung, and Blood Institute; and the President’s Grant-in-Aid Award from the American Academy of Allergy, Asthma, and Immunology. Received for publication November 3, 2003. Accepted for publication in revised form February 12, 2004.
PY - 2004/8
Y1 - 2004/8
N2 - Background: Although there is evidence that contact with mice is associated with IgE-mediated mouse sensitization and mouse specific antibody responses, the exposure-response relationships remain unclear. Objective: To determine whether IgE-mediated mouse sensitization and mouse specific IgG (mIgG) and mIgG4 levels increase with increasing Mus m 1 exposure. Methods: One hundred fifty-one workers at a mouse research and production facility were studied. Exposure assignments were made by linking participants to airborne Mus m 1 concentrations in their respective work areas. Cumulative exposure was estimated by multiplying airborne Mus m 1 concentration by duration of employment. Serum mIgG and mIgG4 levels were quantified by antigen-binding assays, and IgE-mediated mouse sensitization was evaluated by skin prick testing (SPT). Results: Prevalence rates of mouse SPT sensitivity and of high levels of mIgG and mIgG4 were increasingly higher by quintiles of increasing cumulative exposure (P < .01 for SPT, mIgG, and mIgG4). After adjusting for age, sex, and atopy, the log odds ratio (OR) of having positive mouse SPT results was linearly related to cumulative exposure (r2 = 0.87), as was the log OR of having a high mIgG level (r2 = 0.86). Quintile of cumulative exposure was an independent predictor of both SPT sensitivity (OR, 1.7; 95% confidence interval, 1.2-2.5) and a high mIgG level (OR, 1.7; 95% confidence interval, 1.2-2.4). Conclusions: IgE-mediated mouse sensitization and mIgG and mIgG4 levels were related to cumulative exposure in a dose-dependent manner. Thus, strategies to prevent allergy to mice should remain focused on reducing mouse allergen exposure.
AB - Background: Although there is evidence that contact with mice is associated with IgE-mediated mouse sensitization and mouse specific antibody responses, the exposure-response relationships remain unclear. Objective: To determine whether IgE-mediated mouse sensitization and mouse specific IgG (mIgG) and mIgG4 levels increase with increasing Mus m 1 exposure. Methods: One hundred fifty-one workers at a mouse research and production facility were studied. Exposure assignments were made by linking participants to airborne Mus m 1 concentrations in their respective work areas. Cumulative exposure was estimated by multiplying airborne Mus m 1 concentration by duration of employment. Serum mIgG and mIgG4 levels were quantified by antigen-binding assays, and IgE-mediated mouse sensitization was evaluated by skin prick testing (SPT). Results: Prevalence rates of mouse SPT sensitivity and of high levels of mIgG and mIgG4 were increasingly higher by quintiles of increasing cumulative exposure (P < .01 for SPT, mIgG, and mIgG4). After adjusting for age, sex, and atopy, the log odds ratio (OR) of having positive mouse SPT results was linearly related to cumulative exposure (r2 = 0.87), as was the log OR of having a high mIgG level (r2 = 0.86). Quintile of cumulative exposure was an independent predictor of both SPT sensitivity (OR, 1.7; 95% confidence interval, 1.2-2.5) and a high mIgG level (OR, 1.7; 95% confidence interval, 1.2-2.4). Conclusions: IgE-mediated mouse sensitization and mIgG and mIgG4 levels were related to cumulative exposure in a dose-dependent manner. Thus, strategies to prevent allergy to mice should remain focused on reducing mouse allergen exposure.
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U2 - 10.1016/S1081-1206(10)61471-8
DO - 10.1016/S1081-1206(10)61471-8
M3 - Article
C2 - 15328678
AN - SCOPUS:4344704559
SN - 1081-1206
VL - 93
SP - 171
EP - 178
JO - Annals of Allergy, Asthma and Immunology
JF - Annals of Allergy, Asthma and Immunology
IS - 2
ER -