TY - JOUR
T1 - Morphologies of mouse retinal ganglion cells expressing transcription factors Brn3a, Brn3b, and Brn3c
T2 - Analysis of wild type and mutant cells using genetically-directed sparse labeling
AU - Badea, Tudor Constantin
AU - Nathans, Jeremy
N1 - Funding Information:
Supported by the Howard Hughes Medical Institute.
PY - 2011/1/28
Y1 - 2011/1/28
N2 - The mammalian retina contains more than 50 distinct neuronal types, which are broadly classified into several major classes: photoreceptor, bipolar, horizontal, amacrine, and ganglion cells. Although some of the developmental mechanisms involved in the differentiation of retinal ganglion cells (RGCs) are beginning to be understood, there is little information regarding the genetic and molecular determinants of the distinct morphologies of the 15-20 mammalian RGC cell types. Previous work has shown that the transcription factor Brn3b/Pou4f2 plays a major role in the development and survival of many RGCs. The roles of the closely related family members, Brn3a/Pou4f1 and Brn3c/Pou4f3 in RGC development are less clear. Using a genetically-directed method for sparse cell labeling and sparse conditional gene ablation in mice, we describe here the sets of RGC types in which each of the three Brn3/Pou4f transcription factors are expressed and the consequences of ablating these factors on the development of RGC morphologies.
AB - The mammalian retina contains more than 50 distinct neuronal types, which are broadly classified into several major classes: photoreceptor, bipolar, horizontal, amacrine, and ganglion cells. Although some of the developmental mechanisms involved in the differentiation of retinal ganglion cells (RGCs) are beginning to be understood, there is little information regarding the genetic and molecular determinants of the distinct morphologies of the 15-20 mammalian RGC cell types. Previous work has shown that the transcription factor Brn3b/Pou4f2 plays a major role in the development and survival of many RGCs. The roles of the closely related family members, Brn3a/Pou4f1 and Brn3c/Pou4f3 in RGC development are less clear. Using a genetically-directed method for sparse cell labeling and sparse conditional gene ablation in mice, we describe here the sets of RGC types in which each of the three Brn3/Pou4f transcription factors are expressed and the consequences of ablating these factors on the development of RGC morphologies.
KW - Brn3 transcription factors
KW - Dendritic arbor
KW - Mouse
KW - Retina development
KW - Retinal ganglion cell
UR - http://www.scopus.com/inward/record.url?scp=78651508816&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78651508816&partnerID=8YFLogxK
U2 - 10.1016/j.visres.2010.08.039
DO - 10.1016/j.visres.2010.08.039
M3 - Article
C2 - 20826176
AN - SCOPUS:78651508816
SN - 0042-6989
VL - 51
SP - 269
EP - 279
JO - Vision Research
JF - Vision Research
IS - 2
ER -